Transsinaptik Kolera Toksin ve Biotinlenmiş Dekstran Aminler Çift Etiketleme Ardından Pseudorabies'e Virüs ile Periferik Hedefler gelen İzleme

Transsynaptic tracing has become a powerful tool for analyzing central efferents regulating peripheral targets through multi-synaptic circuits. Here we present a protocol that exploits the transsynaptic pseudorabies virus to identify and localize a functional brain circuit, followed by classical tract tracing techniques to validate specific connections in the circuit between identified groups of neurons.