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DOI :
10.3791/50677-v
August 23rd, 2013
Chapters
0:05
Title
2:33
Neutrophil Isolation using Polymorphprep
4:31
Neutrophil Labeling with Calcein AM
5:47
Neutrophil Binding Assay
7:43
Results: Measuring Neutrophil Adhesion to Activated Primary HMVEC under Static Conditions
10:57
Conclusion
1:48
Plating and Maintenance of Human Microvascular Endothelial Cells (HMVEC)
中性粒细胞活化的内皮细胞在感染部位是坚持宿主的炎症反应的一个组成部分。本报告中所述的是中性粒细胞的结合测定法,它允许对在体外初级人类嗜中性粒细胞的定量静态条件下的炎症介质激活内皮细胞的结合。
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