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DOI :
10.3791/50684-v
•
7:10 min
October 8th, 2013
Chapters
0:05
Title
2:07
Preparation of Bacterial Cultures, Tools, and Reagents
3:04
Growth and Preparation of Electrocompetent Bacteria
4:01
Transformation of Electrocompetent Bacteria
4:58
Representative Transformation Results
6:18
Conclusion
电穿孔是用于将DNA导入细菌中称为转化的过程中通常采用的方法。为电感受态细胞的制备传统的协议是耗时且劳动密集型的。本文介绍了一种替代,快速,并为电感受态细胞通过一些实验室目前使用的高效制备方法。
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