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DOI :
10.3791/50734-v
November 13th, 2013
Chapters
0:05
Title
2:35
13C-Labeling of DNA in Metabolically Active Gut Bacteria
3:42
Insect Dissection
5:06
DNA Extraction and Amplification
6:13
CsCl Gradient/DNA Ultracentrifugation
8:27
Processing the CsCl Gradients
9:45
Identifying Metabolically Active Gut Bacteria
11:13
Conclusion
ハスモンヨトウのサキシマスオウノキの腸に関連するアクティブ細菌群集は、ピロシーケンスに結合された安定同位体プロービング(SIP)により決定した。この方法論を使用して、コミュニティ内の代謝的に活性な細菌種の同定は、高分解能かつ高精度に行った。
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