Name: a procedure to study the effect of prolonged food restriction on heroin seeking in abstinent rats
Uploaded: 2013-11-11T20:00:00
Description: Watch this Scientific Journal Video about A Procedure to Study the Effect of Prolonged Food Restriction on Heroin Seeking in Abstinent Rats at JoVE.com

这项工作是由自然科学与工程理事会探索项目（美国：298915）的支持，该基金去RECHERCHE魁北克 - 桑特（CSBN），和加拿大研究教席计划（美国）。

1。动物所有大鼠被视为按照加拿大动物保护协会的指导方针和批准所有的实验程序被授予由Concordia大学动物研究伦理委员会。 <ol><li>获得大鼠在275-300克（男性）或225-250克（女）。房子老鼠成对直到手术在标准的塑料笼中含玉米芯床上用品和碎纸。 </li><li>大鼠维持在一个颠倒光暗周期（上午9:30灯熄灭）。 </li><li>确保食物和水，可自由采食 ，除了在禁欲期。我们使?...

<ol>
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L., Stewart, J., Shaham, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Toward+a+model+of+drug+relapse:+an+assessment+of+the+validity+of+the+reinstatement+procedure.">Toward a model of drug relapse: an assessment of the validity of the reinstatement procedure.</a> Psychopharmacology. 189, 1-16 (2006).</li><li>Zacny, J. P., de Wit, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effects+of+a+restricted+feeding+regimen+on+cigarette+smoking+in+humans.">The effects of a restricted feeding regimen on cigarette smoking in humans.</a> Addict. Behav. 17, 149-157 (1992).</li><li>Fuchs, R. A., Lasseter, H. C., Ramirez, D. R., Xie, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relapse+to+drug+seeking+following+prolonged+abstinence:+the+role+of+environmental+stimuli.+Drug+Discov.+Today+Dis.">Relapse to drug seeking following prolonged abstinence: the role of environmental stimuli. Drug Discov. Today Dis.</a> Models. 5, 251-258 (2008).</li><li>D'Cunha, T. 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这里所描述的过程具有很强的表面效度，因为1）它允许自愿服药及以上药品监督管理部门控制，2）它采用的给药途径是人类共同的吸毒者，3）它反映了（相对）延长药物暴露的时期和禁欲，和4）避免了寻求药物测试是不可或缺的恢复过程，但在人类成瘾者14很少观察到之前的灭绝培训。此外，该过程模型考虑热量限制对觅药5的影响时，被认为是至关重要的一个延长食品的限制?...

其中一个在戒毒治疗中的主要困难是复发率很高，即使在禁欲1,2长时间。在研究人类成瘾者已经确定的环境挑战的重要贡献，如接触到药物相关的离散线索或环境3和应激性生活事件4，复发。被发现有吸毒和渴求的影响的共同的环境挑战是长期的粮食限制。例如，已经报道，慢性热量限制与增加卷烟吸烟5相关联。 &#160;此外，节食的严重程度（限制热量）是积极与使用毒品的年轻女性6患病率增加相关。但是，基本的脑机制尚不完全清楚。 由于涉及到人类的研究，动物模型小时实用和伦理方面的考虑AVE已经发展到确定相关的神经机制。我们以前使用的复职程序7,8展示复牌熄灭觅药大鼠暴露在急性（24-48小时）食物匮乏9,10。然而，恢复过程的一个组成部分是消光周期不是药物成瘾治疗11的共同特征（但参见Epstein 等人12年的恢复过程的有效性进行全面的讨论）。此外，在人类中，只有长期的粮食限制，但不严重的粮食匮乏的饮食操作的具体情况，结果表明，以有效地提高吸毒5,13。 因此，我们提出了“节欲程序”14，允许长期粮食限制觅药的影响的研究。使用这种方法，我们最近展示了h的稳健增强eroin寻求戒断大鼠暴露于轻度限食15 14天。

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			<td height="137" style="height:137px;width:177px;">Silastic tubing (ID 0.02, OD 0.037)</td>
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			<td height="137" style="height:137px;width:177px;">Fixed speed infusion pump (3.3 rpm)</td>
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			<td style="width:252px;">H12-02R-2.9</td>
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			<td height="69" style="height:69px;width:177px;">Retractable lever-rat</td>
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			<td style="width:411px;">Concordia University</td>
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			<td height="69" style="height:69px;width:177px;">System controller 2</td>
			<td style="width:411px;">Coulbourn Instruments</td>
			<td style="width:252px;">SYS CTRL 2</td>
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			<td style="width:411px;">Coulbourn Instruments</td>
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a procedure to study the effect of prolonged food restriction on heroin seeking in abstinent rats

在人类的吸毒者，接触到药物相关线索或以前与吸毒相关的环境中也能戒酒过程中引发复发。此外，各种环境挑战可能加剧这种效果，以及持续增加药物摄入。 我们在这里描述的过程凸显了一个共同的环境挑战，食物限制，对药物的渴求被表示为觅药戒断大鼠的增强的影响。 大鼠植入慢性静脉静脉导管，然后训练按下一个杠杆 四海洛因一段10-12天。继海洛因自我管理相大鼠从操作性条件反射室取出并​​安置在动物护理设施，为期至少14天。当一组是根据不受限制地访问食品（吃饱集团）维护，第二组（FDR组）暴露于英里LD食物限制方案，结果在它们的体重保持在90％的不受限制的体重。在限食14天的大鼠被调回药物的培训环境，以及寻求药物测试消光条件下运行（ 即 。杠杆压力机不会导致海洛因交付）。 这里介绍的方法导致海洛因寻求的高度可靠的隆胸考试当天在食品的限制作用。此外，相比我们之前已经使用了严重的粮食匮乏的操作，目前的程序是更多的临床相关模型的热量限制在寻求药物的影响。此外，它可能是更接近人类生存条件的大鼠不需要寻求药物测试，这是受欢迎的恢复过程的一个组成部分之前，要经过一个灭绝培训阶段。

随着时间的推移体重 ：典型的结果表明，体重在整个海洛因自身给药训练保持稳定。在禁欲，大鼠不受限制地访问州城增加他们的体重，而那些被限制的食物减少他们的体重，以自己的原体重的约90％或吃饱大鼠体重的75-80％（ 图2） 。 输液，在培训活动和非活动杆反应 ：输液和主动杆响应增幅比训练日，而无效的杠杆回应不（ 图3）。?...

Watch this Scientific Journal Video about A Procedure to Study the Effect of Prolonged Food Restriction on Heroin Seeking in Abstinent Rats at JoVE.com

A Procedure to Study the Effect of Prolonged Food Restriction on Heroin Seeking in Abstinent Rats

一个程序，可以让药物在寻求食物限制大鼠强大隆胸的示范说明。继海洛因自身给药训练，大鼠经过一个禁欲时期，在一个无毒品的环境，在此期间，他们是温和的食物限制。觅药，然后在药物相关的环境中进行测试。

一个过程来研究延长食品的限制对海洛因寻求戒断大鼠的影响

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