Name: murine cervical heart transplantation model using a modified cuff technique
Uploaded: 2014-10-12T14:00:00
Description: Watch this Scientific Journal Video about Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique at JoVE.com

The authors declare that they have no competing financial interests.

Dyrene er opstaldet i en barriere patogen fri facilitet. Alle dyr får menneskelig omsorg i overensstemmelse med &quot;principper for Laboratory Animal Care&quot; formuleret af den nationale Selskab for Medicinsk forskning &quot;og&quot; Guide til pleje og anvendelse af forsøgsdyr &quot;udarbejdet af National Academy of Sciences og offentliggjort af National Institutes of Health (NIH publikation nr. 86-23, revideret 1985). Alle forsøg er godkendt af det østrigske ministerium for Uddannelse, Videnskab og Kultur. <...

<ol>
	<li>Brandacher, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tetrahydrobiopterin+compounds+prolong+allograft+survival+independently+of+their+effect+on+nitric+oxide+synthase+activity.">Tetrahydrobiopterin compounds prolong allograft survival independently of their effect on nitric oxide synthase activity.</a> Transplantation. 81, 583-589 (2006).</li><li>Schneeberger, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effect+of+secretory+leukocyte+protease+inhibitor+(SLPI)+on+ischemia/reperfusion+injury+in+cardiac+transplantation.">The effect of secretory leukocyte protease inhibitor (SLPI) on ischemia/reperfusion injury in cardiac transplantation.</a> Am J Transplant. 8, 773-782 (2008).</li><li>Sucher, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=IDO+and+regulatory+T+cell+support+are+critical+for+cytotoxic+T+lymphocyte-associated+Ag-4+Ig-mediated+long-term+solid+organ+allograft+survival.">IDO and regulatory T cell support are critical for cytotoxic T lymphocyte-associated Ag-4 Ig-mediated long-term solid organ allograft survival.</a> JImmunol. 188, 37-46 (2012).</li><li>Yamashita, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heme+oxygenase-1+is+essential+for+and+promotes+tolerance+to+transplanted+organs.">Heme oxygenase-1 is essential for and promotes tolerance to transplanted organs.</a> FASEB J. 20, 776-778 (2006).</li><li>Kienzl, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proteomic+profiling+of+acute+cardiac+allograft+rejection.">Proteomic profiling of acute cardiac allograft rejection.</a> Transplantation. 88, 553-560 (2009).</li><li>Aurora, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Registry+of+the+International+Society+for+Heart+and+Lung+Transplantation:+tenth+official+pediatric+lung+and+heart/lung+transplantation+report--2007.">Registry of the International Society for Heart and Lung Transplantation: tenth official pediatric lung and heart/lung transplantation report--2007.</a> J Heart Lung Transplant. 26, 1223-1228 (2007).</li><li>H&#228;yry, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+allograft+vasculopathy+new+strategies+for+drug+development.">Chronic allograft vasculopathy new strategies for drug development.</a> Transplant Proc. 30, 3989-3990 (1998).</li><li>Hosenpud, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Immune+mechanisms+of+cardiac+allograft+vasculopathy+an+update.">Immune mechanisms of cardiac allograft vasculopathy an update.</a> Transpl Immunol. 1, 237-249 (1993).</li><li>Libby, P., Pober, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+rejection.">Chronic rejection.</a> Immunity. 14, 387-397 (2001).</li><li>Kouwenhoven, E. A., de Bruin, R. W., Heemann, U. W., Marquet, R. L., Ijzermans, J. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Late+graft+dysfunction+after+prolonged+cold+ischemia+of+the+donor+kidney+inhibition+by+cyclosporine.">Late graft dysfunction after prolonged cold ischemia of the donor kidney inhibition by cyclosporine.</a> Transplantation. 68, 1004-1010 (1999).</li><li>Kouwenhoven, E. A., Ijzermans, J. N., de Bruin, R. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Etiology+and+pathophysiology+of+chronic+transplant+dysfunction.">Etiology and pathophysiology of chronic transplant dysfunction.</a> Transpl Int. 13, 385-401 (2000).</li><li>Chen, Z. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+technique+of+cervical+heterotopic+heart+transplantation+in+mice.">A technique of cervical heterotopic heart transplantation in mice.</a> Transplantation. 52, 1099-1101 (1991).</li><li>Huang, X., Chen, D., Chen, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=[A+modified+model+of+cervical+heterotopic+cardiac+transplantation+for+chronic+rejection+research].">[A modified model of cervical heterotopic cardiac transplantation for chronic rejection research].</a> Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 22, 1508-1510 (2008).</li><li>Hasegawa, T., Visovatti, S. H., Hyman, M. C., Hayasaki, T., Pinsky, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heterotopic+vascularized+murine+cardiac+transplantation+to+study+graft+arteriopathy.">Heterotopic vascularized murine cardiac transplantation to study graft arteriopathy.</a> Nat Protoc. 2, 471-480 (2007).</li><li>Wang, C. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Suppression+of+murine+cardiac+allograft+arteriopathy+by+long-term+blockade+of+CD40-CD154+interactions.">Suppression of murine cardiac allograft arteriopathy by long-term blockade of CD40-CD154 interactions.</a> Circulation. 105, 1609-1614 (2002).</li><li>Matsuura, A., Abe, T., Yasuura, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simplified+mouse+cervical+heart+transplantation+using+a+cuff+technique.">Simplified mouse cervical heart transplantation using a cuff technique.</a> Transplantation. 51, 896-898 (1991).</li><li>Tomita, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+technique+of+heterotopic+cervical+heart+transplantation+in+mice.">Improved technique of heterotopic cervical heart transplantation in mice.</a> Transplantation. 64, 1598-1601 (1997).</li><li>Zhou, Y., Gu, X., Xiang, J., Qian, S., Chen, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+comparative+study+on+suture+versus+cuff+anastomosis+in+mouse+cervical+cardiac+transplant.">A comparative study on suture versus cuff anastomosis in mouse cervical cardiac transplant.</a> Exp Clin Transplant. 8, 245-249 (2010).</li><li>Corry, R. J., Winn, H. J., Russell, P. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Primarily+vascularized+allografts+of+hearts+in+mice.+The+role+of+H-2D,+H-2K,+and+non-H-2+antigens+in+rejection.">Primarily vascularized allografts of hearts in mice. The role of H-2D, H-2K, and non-H-2 antigens in rejection.</a> Transplantation. 16, 343-350 (1973).</li><li>Kamada, N., Calne, R. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+liver+transplantation+in+the+rat.+Technique+using+cuff+for+portal+vein+anastomosis+and+biliary+drainage.">Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage.</a> Transplantation. 28, 47-50 (1979).</li><li>Zimmermann, F. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Techniques+for+orthotopic+liver+transplantation+in+the+rat+and+some+studies+of+the+immunologic+responses+to+fully+allogeneic+liver+grafts.">Techniques for orthotopic liver transplantation in the rat and some studies of the immunologic responses to fully allogeneic liver grafts.</a> Transplant Proc. 11, 571-577 (1979).</li><li>Maglione, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Donor+pretreatment+with+tetrahydrobiopterin+saves+pancreatic+isografts+from+ischemia+reperfusion+injury+in+a+mouse+model.">Donor pretreatment with tetrahydrobiopterin saves pancreatic isografts from ischemia reperfusion injury in a mouse model.</a> Am J Transplant. 10, 2231-2240 (2010).</li><li>Heron, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+technique+for+accessory+cervical+heart+transplantation+in+rabbits+and+rats.">A technique for accessory cervical heart transplantation in rabbits and rats.</a> Actapathologica et microbiologica Scandinavica Section A, Pathology. 79, 366-372 (1971).</li></ol>

Afvisning af vaskulariserede allotransplantater medfører et væld af forskellige trin, som kun kan være utilstrækkeligt vurderet ved hjælp af in vitro-modeller. Fænomener som sensibilisering af modtagere antigenbehandling i sekundære lymfoide organer, men også differentiering og proliferation af immun-kompetente celler er bedre karakteriserede in vivo. Dyremodeller er dermed det ideelle værktøj til translationel forskning 16. Musemodeller udgør stadig den gyldne standard i grundlæ...

Hjertetransplantation repræsenterer terapi valg for patienter, der lider af forskellige slutstadiet hjertesygdomme. Fremskridt inden for kirurgiske teknikker, mere effektiv forebyggelse af infektioner, og nye immunosuppressive regimer resulterede i markant forbedret resultat af organtransplantation 6. Imidlertid har langsigtede graft overlevelse ikke forbedret strengt i de seneste år 7. Kronisk afstødning, kendetegnet ved transplantation åreforkalkning fortsætter med at være en væsentlig hindring for langsigtede graft overlevelse 8-11. Modellen for heterotopisk hjerte transplantation i mus giver et vigtigt og brugbart redskab til analyse af immunologisk mekanisme under akut såvel som kronisk afstødning 12-15. Til dato er den hyppigste transplantation model er stadig den abdominale mus hjerte transplantation ved hjælp af sutur teknik. Den opadgående aorta af donor hjerte er anastomoseret til enbdominal aorta og lungepulsåren er anastomoseret til modtagerens vena cava inferior. Meget af den mikrokirurgisk sværhedsgrad af suturen model bygger på den lille størrelse af de fartøjer, som har suturerede 16,17. I modsætning til suturen model hjertet er placeret i halsregionen af ​​modtageren, hvor den eksterne halsvene er anastomeres lungepulsåren og fælles carotidarterie aorta donoren. Begrundelsen for udviklingen af ​​livmoderhalskræft hjerte transplantation model ved hjælp af manchetten teknik var at have en dyremodel, som gør det muligt at opnå høj succesrate med grundlæggende mikrokirurgiske færdigheder, som vil give bred anvendelse af denne model. De største fordele ved denne metode er den betydeligt mindre forekommende anastomose relaterede komplikationer, såsom blødninger og thrombose i forhold til suturen model 18.

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			<td>Yasargil Clip Mini Permanent 7mm</td>
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			<td></td>
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			<td>Micro vessel clip</td>
			<td>S &#38; T</td>
			<td>B1 00396 V</td>
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			<td>Microscissor</td>
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			<td rowspan="2">Vesseldilatator</td>
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			<td>Microforceps</td>
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			<td>Dumont 11271- 30</td>
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			<td>Clipapplicator</td>
			<td>S &#38; T</td>
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			<td>Microvessel clip</td>
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murine cervical heart transplantation model using a modified cuff technique

<html> Musemodeller er af særlig interesse for forskning, idet en lang række monoklonale antistoffer og kommercielt defineret indavlede og knockout-stammer er tilgængelige til at udføre mekanisk in vivo-undersøgelser. Mens hjertetransplantation modeller med en sutur teknik blev først succes udviklet i rotter blev oversættelsen til en lige så udbredt brugt museækvivalenten aldrig opnået på grund af den tekniske kompleksitet mikrokirurgisk procedure. I modsætning hertil blev der ikke sutur manchet teknikker, også oprindeligt udviklet i rotter med succes tilpasses til anvendelse i mus 1-3. Denne teknik til revaskularisering involverer to store skridt I) krænge modtageren fartøj over en polyethylen manchet; II) trækker donorfartøjets over tidligere everteret modtager fartøj og holde den på plads med en perifer slips. Dette sikrer en kontinuitet endothellaget, kort driftstid og meget høje åbentstående kar 4. t &quot;&gt; Brug af denne teknik til vaskulære anastomose udførte vi mere end 1.000 cervikale hjertetransplantationer med en samlet succesrate på 95%. Til arteriel indstrømning den fælles carotidarterie og den proksimale aortabuen blev anastomoseret resulterer i en retrograd perfusion af det transplanterede hjerte. for venøs dræning lungepulsåren af transplantatet blev anastomoseret med den eksterne halsvene modtageren 5. Heri giver vi yderligere detaljer ved denne teknik at supplere videoen.

Figur 2 viser repræsentative data overlevelse af hjerteallografter fra BALB / c-donorer efter transplantation i fuldt MHC-fejlparrede C57BL / 6 modtagere. Hjerter bliver afvist på dag 7 ± 1 efter transplantation. Finger palpering af det transplanterede allograft er en følsom metode til påvisning af den tidsafhængige løbet af allotransplantatafstødning. Alle syngene hjerte transplantationer foretaget i vores serie overlevede på ubestemt tid (&gt; 150 dage). <p class="jove_content" fo:keep-to...

Watch this Scientific Journal Video about Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique at JoVE.com

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

Den murine cervikal hjerte transplantation model er velegnet til immunologisk samt iskæmisk reperfusionsbeskadigelse studier. Vi modificerede procedure bruger en ikke-sutur manchet teknik og gennemført mere end 1.000 vellykkede transplantationer med denne tilgang. Heri giver vi yderligere detaljer ved denne teknik at supplere videoen.

Muse Livmoderhalskræft Heart transplantation model ved hjælp af en modificeret Cuff Teknik

Mouse models are of special interest in research since a wide variety of monoclonal antibodies and commercially defined inbred and knockout strains are ...

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The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies ...

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