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Секс Стратифицированная нейронов культур для изучения ишемических: Подготовка гибель клеток

DOI :

10.3791/50758-v

December 9th, 2013

December 9th, 2013

11,888 Views

1Department of Anesthesiology, University of Colorado School of Medicine, 2Oregon National Primate Research Center, Oregon Health & Science University, 3Department of Pharmacology, University of Colorado School of Medicine

Primary disassociated embryonic hippocampal neuronal cultures are useful for investigating the signaling mechanisms involved in neuron death. Sexing the embryos before the isolation and dissociation of the hippocampus allows the preparation of separate male and female cultures, which enables the researcher to identify and investigate sex-specific cell signaling.

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Sex Stratified Neuronal Cultures

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