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DOI :
10.3791/50762-v
•
16:11 min
September 16th, 2013
Chapters
0:05
Title
1:41
Cell Lysis
6:29
Extract Clarification
8:32
Experimental Execution of a TX-TL Reaction
10:40
Results: Assessment of TX-TL Cell-free Expression System
15:56
Conclusion
这次为期五天的协议列出了所有步骤,设备,以及用于创建和运行基于一个有效的内源性大肠杆菌从头TX-TL无细胞表达系统必要的补充软件。用试剂,该协议需要8个小时或更短设置一个反应,收集和处理数据。
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