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DOI :
10.3791/50772-v
September 23rd, 2013
Chapters
0:05
Title
3:48
Surface Preparation
5:07
Monitoring On-chip Capture-cycloaddition of Functionalized Molecules
8:27
Measuring the Binding of FKBP12 to Immobilized AP1497
10:49
Results: Real-time Monitoring of On-chip Small Molecule Immobilization by Surface Plasmon Resonance
1:48
Preparation of GST and Nanoparticle Conjugates
6:26
Monitoring Immobilization Density and Determination of Cycloaddition Rates
13:51
Conclusion
우리는 순차적으로 온 - 칩 bioorthogonal 사이클로 화학 및 항체 - 항원 캡처를 사용하여 작은 분자의 빠른 가역적 고정화 및 표면 플라스 몬 공명 (SPR) 연구를위한 기능화 나노 입자 어셈블리에 대한 방법을 제시한다.
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