Name: a rapid protocol for integrating extrachromosomal arrays with high transmission rate into the c. elegans genome
Uploaded: 2013-12-09T20:00:00
Description: Watch this Scientific Journal Video about A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome at JoVE.com

Authors would like to acknowledge Claire Lecroisey and Laurent S&#233;galat for generating nonintegrated Pdyc-1::dyc-1::gfp; Pmyo-2::dsRed and Pmyo-3::gfp transgenic strains. We thank Andy Fire for the gift of the Pmyo-2::dsRed plasmid and Pamela Hoppe for the gift of the Pmyo-3::gfp plasmid. This work was founded by the French National Center of Scientific Research (CNRS) and the Claude Bernard University of Lyon.

1. Culturing Transgenic Nematodes Prior to&#160;Integration
Transgenic lines can be generated as described in1. These lines usually contain a coinjection marker plasmid leading either to the expression of a fluorescent protein or to a specific behavioral or morphological phenotype at a given development stage1,3. This integration protocol can be applied to strains expressing fluorescent or nonfluorescent coinjection markers. In the example to follow, a strain with a fluores...

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The protocol described here provides an efficient method to integrate extrachromosomal transgenic arrays into the genome of C. elegans. Using different nonintegrated lines, which transmitted the same transgene Pdyc-1::dyc-1::gfp (pKG45) at different rates (10-15%, 50-60%, and &#62;80%), we showed that the number of integrated strains recovered after UV irradiation is dependent of the initial level of transmission of the nonintegrated line (Table 2). Therefore, transgenes are easier to i...

Transgenes are extensively used in C. elegans for a large range of applications. Transgenic strains are typically generated by DNA injection into the syncytial hermaphrodite gonad1,2. The DNA carrying the transgene of interest is coinjected along with plasmids encoding coinjection markers1,3,4. Coinjection markers are established transgenes leading either to the expression of a fluorescent protein or to a specific behavioral or morphological phenotype. The injected DNAs rearrange to form multi copy extrachromosomal arrays; thus the transgene of interest and the coinjection marker are transmitted together5,4. Transgenic lines are selected in the F2 generation of injected animals by following the phenotype induced by the expression of the coinjection marker (fluorescence or specific phenotype)1. Transgenic lines exhibit partial and variable transmission rates of the extrachromosomal arrays to the next generation. Depending on the strain, 10-100% (100% being a rare event) of the animals inherit extrachromosomal arrays. In addition, transgenic animals are mosaic and express the transgene in a variable number of cells. This mosaicism is likely to be correlated to the transmission rate to the next generation. Indeed, in both germ line and somatic cells the transmission rate depends on the segregation of the extrachromosomal arrays during cell divisions. One way to avoid this issue is to integrate the transgene into the genome. Classically, the integration of a transgene into a chromosome relies on irradiation (ultraviolet or gamma) of transgenic worms carrying extrachromosomal arrays3. Briefly, 50-100 hermaphrodite animals are irradiated at the L4 larval stage. In the F1 generation 200-800 transgenic animals are selected and individually cultured onto plates. According to Mendelian segregation, transgenic F1 animals carrying a heterozygous integration of the transgene into the genome would produce 1/2 of transgenic descendants that are heterozygous for the integrated array, 1/4 of transgenic descendants being homozygous for the integrated array, and 1/4 of descendants that have not integrated the array (Figure 1). Thus, plates containing the F2 generation are visually screened for &#8805;75% transgenic animals as observed by the expression of the coinjection marker. About 1/3 of the F2 animals selected for being integrant candidates are assumed to be homozygous for the integrated array and to produce 100% of homozygous F3 animals. Hence, from each selected plate, three to eight transgenic F2 animals are individually cultured and plates with 100% of transgenic descendants are selected. Next, eight F3 animals are individually cultured to confirm the 100% inheritance of the transgene.
The main disadvantages of this method are that
<ol type="i">
	<li>it requires a visual screen of several hundred F1 plates for &#8805;75% transgenic F2 progeny, which is time consuming due to the variable and unpredictable percentage of transmission of nonintegrated extrachromosomal arrays. Alternatively, to avoid the screening of F1 plates several subsequent starvation steps can be performed after irradiation of P0 animals; followed by picking 100 transgenic animals and screening for 100% transgenic progeny3.</li>
	<li>it is not adapted for strains exhibiting a percentage of extrachromosomal array transmission higher than 60% as this percentage is difficult to distinguish this percentage from 75%.</li>
	<li>as only strains with a relatively low transmission rate can be used, a high number of irradiated transgenic P0 animals is necessary as well as the observation of numerous F1 and F2 animals, which is highly time consuming and requires a considerable number of plates.</li>
</ol>
The irradiation of worms presumably induces double-strand breaks in the DNA and integration of extrachromosomal arrays into chromosomes occurs during DNA repair. Thus, it is likely that chances to obtain successful irradiation mediated integration are directly proportional to the number of germ line cells carrying an extrachromosomal array. Strains with a high transmission rate may contain more transgenic DNA arrays than strains with a low transmission rate as suggested in2, and exhibit a higher number of germ line cells carrying extrachromosomal arrays. Thus, we reasoned that in highly transmitting lines, transgenes might be easier to integrate than in lines with a low transmission rate. However, the standard protocol excludes the possibility of integrating transgenes with high transmission frequency as the percentage of transgenic animals with a nonintegrated extrachromosomal array can hardly be distinguished from that of the progeny of a F1 animal carrying a heterozygous integration of the transgene into the genome (Figure 1).
Here we show that for a particular transgene, the number of integrated lines recovered after irradiation with ultraviolet (UV) light is dependent on the initial percentage of transmission of the nonintegrated array. We present an improved protocol for UV irradiation-mediated transgene integration that is particularly relevant to lines exhibiting a high rate of transgene array transmission.

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		<tr height="41">
			<td height="41" style="height:41px;width:167px;">Agar</td>
			<td style="width:173px;">Prolabo</td>
			<td style="width:181px;">20768.292</td>
			<td style="width:344px;">for NGM (nematode growth medium) preparation (6,7)</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">NaCl</td>
			<td style="width:173px;">Chimie-Plus</td>
			<td style="width:181px;">40045</td>
			<td style="width:344px;">for NGM preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Bacto-peptone</td>
			<td style="width:173px;">BD</td>
			<td style="width:181px;">211820</td>
			<td style="width:344px;">for NGM preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">KH2PO4</td>
			<td style="width:173px;">Roth</td>
			<td style="width:181px;">3904-1</td>
			<td style="width:344px;">for NGM preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">K2HPO4</td>
			<td style="width:173px;">Prolabo</td>
			<td style="width:181px;">26930.293</td>
			<td style="width:344px;">for NGM preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">MgSO4</td>
			<td style="width:173px;">Roth</td>
			<td style="width:181px;">P027.2</td>
			<td style="width:344px;">for NGM preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">CaCl2</td>
			<td style="width:173px;">Prolabo</td>
			<td style="width:181px;">22317.297</td>
			<td style="width:344px;">for NGM preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Cholesterol</td>
			<td style="width:173px;">Rectapur</td>
			<td style="width:181px;"></td>
			<td style="width:344px;">for NGM preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">LB-broth</td>
			<td style="width:173px;">Sigma</td>
			<td style="width:181px;">L3022</td>
			<td style="width:344px;">for culture of OP50 bacteria</td>
		</tr>
		<tr height="27">
			<td height="27" style="height:27px;"></td>
			<td></td>
			<td></td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;"></td>
			<td></td>
			<td></td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;"></td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="28">
			<td height="28" style="height:28px;"></td>
			<td></td>
			<td></td>
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		</tr>
		<tr height="27">
			<td height="27" style="height:27px;">EQUIPMENT</td>
			<td></td>
			<td></td>
			<td></td>
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			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Name of Equipment</td>
			<td>Company</td>
			<td>Catalogue number</td>
			<td>Comments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">U.V. cross-linker</td>
			<td>Fischer Scientific</td>
			<td></td>
			<td>Wavelength 254 nm</td>
		</tr>
		<tr height="54">
			<td height="54" style="height:55px;width:167px;">Microscope SteREO Discovery V8</td>
			<td>Carl Zeiss, Inc.</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Petri dishes, 60 mm</td>
			<td>CML</td>
			<td>BPS55E6</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Platinium wire 0.25 mm</td>
			<td>Alfa Aesar</td>
			<td>7440-06-4</td>
			<td>To make a pick for worms (7)</td>
		</tr>
	</tbody>
</table>

a rapid protocol for integrating extrachromosomal arrays with high transmission rate into the c. elegans genome

Microinjecting DNA into the cytoplasm of the syncytial gonad of Caenorhabditis elegans is the main technique used to establish transgenic lines that exhibit partial and variable transmission rates of extrachromosomal arrays to the next generation. In addition, transgenic animals are mosaic and express the transgene in a variable number of cells. Extrachromosomal arrays can be integrated into the C. elegans genome using UV irradiation to establish nonmosaic transgenic strains with 100% transmission rate of the transgene. To that extent, F1 progenies of UV irradiated transgenic animals are screened for animals carrying a heterozygous integration of the transgene, which leads to a 75% Mendelian transmission rate to the F2 progeny. One of the challenges of this method is to distinguish between the percentage of transgene transmission in a population before (X% transgenic animals) and after integration (&#8805;75% transgenic F2 animals). Thus, this method requires choosing a nonintegrated transgenic line with a percentage of transgenic animals that is significantly lower than the Mendelian segregation of 75%. Consequently, nonintegrated transgenic lines with an extrachromosomal array transmission rate to the next generation &#8804;60% are usually preferred for integration, and transgene integration in highly transmitting strains is difficult. Here we show that the efficiency of extrachromosomal arrays integration into the genome is increased when using highly transmitting transgenic lines (&#8805;80%). The described protocol allows for easy selection of several independent lines with homozygous transgene integration into the genome after UV irradiation of transgenic worms exhibiting a high rate of extrachromosomal array transmission. Furthermore, this method is quite fast and low material consuming. The possibility of rapidly generating different lines that express a particular integrated transgene is of great interest for studies focusing on gene expression pattern and regulation, protein localization, and overexpression, as well as for the development of subcellular markers.

Integrations of transgenes in two different lines transmitting extrachromosomal arrays at about the same frequency (50-60%) were performed using the standard and the improved protocols (Table 1). The standard protocol allowed the recovery of three independent integrated lines after UV irradiation of 91 transgenic L4 animals, while the improved protocol allowed the recovery of one integrated line after UV irradiation of 30 transgenic L4 animals. This suggests that the efficiency of integration is similar ...

Watch this Scientific Journal Video about A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome at JoVE.com

A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome

This protocol describes a rapid and low material consuming procedure for the integration of transgenic extrachromosomal arrays into the Caenorhabditis elegans genome using ultra violet (UV) irradiation. Furthermore, this protocol is particularly well suited for transgenic lines that transmit extrachromosomal arrays at a high rate.

A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome

Microinjecting DNA into the cytoplasm of the syncytial gonad of Caenorhabditis elegans is the main technique used to establish transgenic lines that ...

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