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DOI :
10.3791/50783-v
February 12th, 2014
Chapters
0:05
Title
1:19
Preparing Dishes with EM Grids
3:43
Preparing and Plating Primary Neurons on EM Grids
4:45
Vitrifying Neurons on EM Grids
8:05
Results: Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography
9:32
Conclusion
以保留神经流程超微结构分析,我们描述了一种协议,用于在电子显微镜网格后用快速冷冻原代神经元的电镀,得到的样品悬浮在一层玻璃的冰。这些样本可以检查与一个低温电子显微镜可视化结构在纳米尺度。
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