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DOI :
10.3791/50821-v
October 15th, 2013
Chapters
0:05
Title
1:27
Depletion and Isolation of PBMCs via Ficoll Separation
2:08
Antibody Blockade and Antigen-Specific Stimulation of Cells
2:46
Collection and Analysis of Supernatant
3:15
Collection and Phenotypic Analysis of Cells via Flow Cytometry
4:09
Analysis of Cells via qRT-PCR
5:15
Adaptation for Intracellular Cytokine Staining at 48 Hours
5:48
Adaptation for Sorting Cells
6:55
Results: HIV-specific CD4 T Cell Effector Function
9:03
Conclusion
हम एचआईवी विशिष्ट सीडी 4 टी कोशिकाओं द्वारा cytokine स्राव के नियमन में immunoregulatory रास्ते की भूमिका की जांच के लिए इन विट्रो परख विकसित की है.
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