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DOI :
10.3791/50823-v
January 6th, 2014
Chapters
0:05
Title
2:01
Collagen Coating Transwells
3:25
Seeding Collagen Coated Transwells with Epithelial Cells
4:30
Preparation of Epithelial Cell Layers for Migration Assay
6:01
Neutrophil Trans-epithelial Migration Assay
11:27
Results: Assessing Neutrophil Trans-epithelial Migration
13:23
Conclusion
粘膜細菌感染に応答して好中球トランス上皮移動は、上皮損傷および臨床疾患に寄与する。この現象を調整する分子機構の解明に向けた研究を容易にするために、トランスウェルフィルター上で成長した病原体、ヒト好中球、偏光ヒト上皮細胞層を組み合わせた in vitro モデルが開発されました。
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