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The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

DOI :

10.3791/50824-v

November 13th, 2013

November 13th, 2013

13,805 Views

1Department of Craniofacial Development and Stem Cell Biology, and Department of Orthodontics, Dental Institute, Guy's Hospital, UK, King's College London, 2Department of Pediatric Dentistry and Orthodontics, College of Dentistry, King Saud University, Kingdom of Saudi Arabia

Here we detail a method to culture tooth germs in mandible slices using a tissue chopper. This method allows unique access to the tooth during development, providing excellent opportunity for manipulation and lineage tracing, not available using more traditional culture methods.

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