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A Novel Ex vivo Culture Method for the Embryonic Mouse Heart

DOI :

10.3791/50359-v

May 24th, 2013

May 24th, 2013

12,915 Views

1McAllister Heart Institute, University of North Carolina at Chapel Hill

Developmental studies in the mouse are hampered by the inaccessibility of the embryo during gestation. To promote the long-term culture of the embryonic heart at late stages of gestation, we developed a protocol in which the excised heart is cultured in a semi-solid, dilute Matrigel.

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