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Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

DOI :

10.3791/50826-v

December 21st, 2013

December 21st, 2013

7,172 Views

1Developmental Biology Institute of Marseille-Luminy (IBDML), Aix Marseille University, 2Campus de la Timone, European Research Center for Medical Imaging (CERIMED)

We describe a method for implanting and maintaining glass windows over the exposed spinal cords of adult mice for studying experimental autoimmune encephalomyelitis. These windows provide chronic optical access to the spinal cord for monitoring cell dynamics at the subcellular level in live animals using two-photon microscopy.

Tags

Keywords Intravital Microscopy

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