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DOI :
10.3791/50830-v
October 11th, 2013
Chapters
0:05
Title
1:37
Infection of Mammalian Cells with the Recombinant Modified Vaccinia Ankara Virus Expressing the Bacteriophage T7 RNA Polymerase (MVA-T7)
2:40
Transfection of Mammalian Cells
3:48
Co-culture of Mammalian Cells with Avian Cells
5:22
Infection of Chicken Embryonated Eggs
6:31
Harvest of Allentoic Fluid from Infected Chicken Embryonated Eggs
7:52
Hemagglutination (HA) Assay
9:03
Results: Transfection and MVA-T7 Infection Efficiencies and Hemagglutination Assay
10:33
Conclusion
뉴캐슬 질병 바이러스 (NDV)는 광범위 중에서도, 백신 및 치료를위한 새로운 벡터를 개발하기 위해 지난 몇 년 동안 연구되어왔다. 이 연구로 인해 이러한 우리가 여기에서 설명하는 것과의 cDNA에서 재조합 바이러스를 구출하는 기술로 가능했습니다.
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