Name: ultrasonic assessment of myocardial microstructure
Uploaded: 2014-01-14T13:00:00
Description: Watch this Scientific Journal Video about Ultrasonic Assessment of Myocardial Microstructure at JoVE.com

我们非常感谢哈佛医学院/布里格姆和妇女医院心血管生理学核心实验室提供的资源。这项工作的部分资金来自国家卫生研究院赠款HL088533、HL071775、HL093148和HL099073（RL）。MB 是美国心脏协会创始人联盟博士后奖学金的获得者。KU 是美国心脏协会创始人附属博士后奖学金的获得者。SC 得到了埃里森基金会的奖励。

1. 分析图像的准备
<ol><li>在半轴视图中获取穆林或人类超心电图 B 模式图像。根据通常的做法，调整时间收益补偿设置和传输焦点的放置，以优化 LV 和其他心脏结构在参数视图中的可视化。确保以 DICOM 文件格式保存所有图像。标准化的图像视图将推理左心室壁置于框架底部。框架必须显示整个左心室心肌和心肌。分辨率必须足够高，以划定左心室的腹腔边界、心肌壁和心内壁。丢弃具有过量辍...

<ol>
	<li>Yamada, S., Komuro, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Integrated+backscatter+for+the+assessment+of+myocardial+viability.">Integrated backscatter for the assessment of myocardial viability.</a> Curr. Opin. Cardiol. 21, 433-437 (2006).</li><li>Mimbs, J. W., O'Donnell, M., Bauwens, D., Miller, J. W., Sobel, B. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+dependence+of+ultrasonic+attenuation+and+backscatter+on+collagen+content+in+dog+and+rabbit+hearts.">The dependence of ultrasonic attenuation and backscatter on collagen content in dog and rabbit hearts.</a> Circ. 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A videodensitometric study.</a> Eur. Heart. J. 17, 472-479 (1996).</li><li>Komuro, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sensitive+detection+of+myocardial+viability+in+chronic+coronary+artery+disease+by+ultrasonic+integrated+backscatter+analysis.">Sensitive detection of myocardial viability in chronic coronary artery disease by ultrasonic integrated backscatter analysis.</a> J. Am. Soc. Echocardiogr. 18, 26-31 (2005).</li><li>Ciulla, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Echocardiographic+patterns+of+myocardial+fibrosis+in+hypertensive+patients:+endomyocardial+biopsy+versus+ultrasonic+tissue+characterization.">Echocardiographic patterns of myocardial fibrosis in hypertensive patients: endomyocardial biopsy versus ultrasonic tissue characterization.</a> J. Am. Soc. 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我们描述图像分析算法的协议，该算法量化声像信号强度分布，进而提供心肌微观结构的替代测量。协议的标准化功能，包括投资回报率和参考区域的选择、大小和定位，有助于最大限度地减少用户和基于主题的变异性。我们证明，当应用于末期舒张单帧回声心电图图像时，该算法可以适当区分正常心肌与暴露在后负荷应力中的心肌。
该协议详细说明了如何使用开源图像J软?...

心电图是一种广泛访问的成像方式，通常用于非侵入性地描述和量化心脏结构和功能的变化。对心脏组织的超声波评估可以包括在特定时间点以及心脏周期过程中分析特定感兴趣区域内的背散射信号强度。先前的研究表明，测得声像信号强度可以识别心肌纤维混乱、可行与不可行心肌组织以及间质纤维化1-3的基本存在。我们称心肌"微结构"为组织结构，可以使用声学分析进行特征，超越毛额和形态的线性测量。因此，对声像信号强度的分析用于评估心肌组织在肥大和扩张性心肌病4，5，慢性冠状动脉疾病6，7和高血压心脏病8，9的设置的微结构变化。然而，先前建立的技术主要依赖于反散射信号强性的综合值或平均值，这可能容易受到随机噪声5、低帧速率10的别名数据以及基于周期变异11的算法的时间延迟的影响。
本文介绍了使用超声波图像分析算法的方法，该算法从以前的方法延伸至过去：此算法侧重于用于图像分析的单个末端舒张框架，并说明从给定心肌样本中提取的信号强度值的完整分布。该算法使用围网作为帧内参考12，13，可重复量化声学信号强度分布的变化，并提供心肌微观结构的增强替代测量。在分步协议中，我们描述了在选定的感兴趣区域内准备用于使用的图像、取样感兴趣区域和处理数据的方法。我们还展示了应用该算法从小鼠和人类那里获得的回声心电图图像的代表性结果，这些图像可对左心室的后负荷应力进行可变暴露。

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			<td height="21" style="height:21px;width:216px;">ImageJ v 1.46</td>
			<td style="width:268px;">NIH (Bethesda, MD)</td>
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			<td style="width:167px;">open access software</td>
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			<td height="21" style="height:21px;">Power ShowCase</td>
			<td style="width:268px;">Trillium Technology (Ann Arbor, MI)</td>
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			<td>commercial software</td>
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ultrasonic assessment of myocardial microstructure

心电图是一种广泛访问的成像方式，通常用于非侵入性地描述和量化心脏结构和功能的变化。对心脏组织的超声波评估可以包括对特定感兴趣区域内的背散射信号强度的分析。以前建立的技术主要依赖于反散射信号强性的集成值或平均值，这可能容易受到基于周期变化的算法的低帧速率和算法时间延迟的别名数据的变异性的影响。在此，我们描述了一种基于超声波的成像算法，该算法从以前的方法扩展到单个图像帧，并说明从给定心肌样本中提取的信号强度值的完整分布。当应用于具有代表性的鼠标和人类成像数据时，该算法区分有或不暴露于慢性后置阻力的受试者。该算法提供了心肌微观结构的增强替代测量，可使用开放访问图像分析软件执行。

信号强度分析主要包括4个主要步骤（图1），包括：1）图像选择和格式，2）采样投资回报率和参考区域，3）算法应用，4）处理最终值，以产生心肌与心肌强度比。投资回报率的选择和大小标准化，以限制互用器以及内部使用者变异性（图 2）。每个投资回报率的定位也标准化，针对每个主体的解剖结构，以限制主体间和主体内部的变异性。
作为心?...

Watch this Scientific Journal Video about Ultrasonic Assessment of Myocardial Microstructure at JoVE.com

Ultrasonic Assessment of Myocardial Microstructure

回声心动图通常用于非侵入性地描述和量化心脏结构和功能的变化。我们描述了一种基于超声波的成像算法，该算法提供了心肌微观结构的增强替代测量，并且可以使用开放访问图像分析软件执行。

心肌微观结构超声波评估

Echocardiography is a widely accessible imaging modality that is commonly used to noninvasively characterize and quantify changes in cardiac structure and ...

Research

JoVE Journal

Medicine

Magnetic Resonance Derived Myocardial Strain Assessment Using Feature Tracking

磁共振衍生的心肌劳损评估使用特征跟踪

Purpose: An accurate and practical method to measure parameters like strain in myocardial tissue is of great clinical value, since it has been shown, that ...

科研

医学

Acute Myocardial Infarction in Rats

大鼠急性心肌梗死

With heart failure leading the cause of death in the USA (Hunt), biomedical research is fundamental to advance medical treatments for cardiovascular ...

Assessment of Cardiac Function and Myocardial Morphology Using Small Animal Look-locker Inversion Recovery (SALLI) MRI in Rats

Assessment of Cardiac Function and Myocardial Morphology Using Small Animal Look-locker Inversion Recovery SALLI MRI in Rats

使用小动物看更衣室反转恢复（SALLI）的MRI大鼠心脏功能和心肌形态学评估

Small animal magnetic resonance  imaging is an important tool to study cardiac function and changes in myocardial  tissue. The high heart rates of small ...

MRI and PET in Mouse Models of Myocardial Infarction

MRI和PET心肌梗死小鼠模型

Myocardial infarction is one of the leading causes of death in the Western world. The similarity of the mouse heart to the human heart has made it an ...

Myocardial Infarction and Functional Outcome Assessment in Pigs

心肌梗死和功能预后评估的猪

Introduction of newly discovered cardiovascular therapeutics into first-in-man trials depends on a strictly regulated ethical and legal roadmap. One ...

Primary Outcome Assessment in a Pig Model of Acute Myocardial Infarction

急性心肌梗死的猪模型主要成果评估

Mortality after acute myocardial infarction remains substantial and is associated with significant morbidity, like heart failure. Novel therapeutics are ...

Transthoracic Speckle Tracking Echocardiography for the Quantitative Assessment of Left Ventricular Myocardial Deformation

经胸斑点跟踪超声心动图左室心肌变形的定量评价

The value of conventional echocardiography is limited by differences in inter-individual image interpretation and therefore largely dependent on the ...

Histological Quantification of Chronic Myocardial Infarct in Rats

大鼠慢性心肌梗塞的组织学定量分析

Myocardial infarction is defined as cardiomyocyte death due to prolonged ischemia; an inflammatory response and scar formation (fibrosis) follow the ...

3D Whole-heart Myocardial Tissue Analysis

3D全心脏心肌组织分析

Cardiac regenerative therapies aim to protect and repair the injured heart in patients with ischemic heart disease. By injecting stem cells or other ...

Ultrasonic-augmented Primary Adult Fibroblast Isolation

超声波增强原成人成纤维细胞隔离

Primary adult fibroblasts have become an important tool to study fibrosis, fibroblast interactions and inflammation in all body tissues. Since primary ...