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DOI :
10.3791/50856-v
•
12:43 min
December 6th, 2013
Chapters
0:05
Title
1:38
Dissociation of Human Embryonic Stem Cells (hESC) Bulk Cultures into Single Cells
2:44
Immunofluorescent Staining of Cell Surface Antigens TG30 and GCTM-2
5:02
Post-FACS Culture of TG30/GCTM-2 Cell Subfractions
7:46
Enrichment of Pluripotent hESC from TG30Hi-GCTM-2Hi Cells
11:28
Conclusion
我们描述了使用单克隆抗体TG30(CD9)和GCTM-2通过荧光活性细胞分选(FACS)联合检测细胞表面抗原,用于识别和丰富活人胚胎干细胞(hESC),使用阳性选择,以及使用负选择清除混合细胞群中的hESC。
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