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DOI :
10.3791/50856-v
•
12:43 min
December 6th, 2013
Chapters
0:05
Title
1:38
Dissociation of Human Embryonic Stem Cells (hESC) Bulk Cultures into Single Cells
2:44
Immunofluorescent Staining of Cell Surface Antigens TG30 and GCTM-2
5:02
Post-FACS Culture of TG30/GCTM-2 Cell Subfractions
7:46
Enrichment of Pluripotent hESC from TG30Hi-GCTM-2Hi Cells
11:28
Conclusion
我々は、正の選択を用いた生きたヒト胚性幹細胞(hESC)の同定および濃縮のための蛍光活性化細胞分類(FACS)を介した細胞表面抗原の複合検出のためのモノクローナル抗体TG30(CD9)およびGCTM-2の使用と、混合細胞集団からのhESCCをパージするための否定的選択の使用について述べた。
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