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DOI :
10.3791/50883-v
January 12th, 2014
Chapters
0:05
Title
2:05
Transfecting COS7 Cells
5:26
In ovo Electroporation of pHluorin-PlexinA1 Construct
8:20
Expression of pHluorin-PlexinA1
9:26
Conclusion
我们在这里描述了使用pH敏感的绿色荧光蛋白变种,pHluorin,来研究在细胞表面贩运的轴向制导受体的间歇性时间动力学。pHluorin标记的受体在细胞培养和 体内都表达,使用小鸡胚胎的电波。
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