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DOI :
10.3791/50884-v
February 11th, 2014
Chapters
0:05
Title
1:45
Spinal Cord Dissection
5:03
FP Culture and FP Conditioned Medium Harvesting
6:35
Commissural Neuron Dissection and Treatments
8:15
Results: Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals
8:58
Conclusion
フロア·プレートは、脊髄を現像前駆細胞への拡散信号を提供し、ニューロン分化の重要な構造である。我々は、床プレート馴化培地を生成するために、新鮮な脊髄組織に適用し、そして生化学ことにより、目的のタンパク質上の影響を評価するための方法を記載している。
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