Name: microwave-assisted functionalization of poly(ethylene glycol) and on-resin peptides for use in chain polymerizations and hydrogel formation
Uploaded: 2013-10-29T18:30:00
Duration: 15 min 33 s
Description: Watch this Scientific Journal Video about Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation at JoVE.com

इस कार्य / रोचेस्टर विश्वविद्यालय और हड्डी रोग अनुसंधान और शिक्षा फाउंडेशन / Musculoskeletal प्रत्यारोपण फाउंडेशन (oref से डा. डेनिएल बेनोइट के लिए प्रदान शुरू हुआ धन से, एक हॉवर्ड ह्यूजेस मेड में-ग्रैड फैलोशिप (AVH) के हिस्से में वित्त पोषित किया गया MTF). लेखकों को अपने उपकरणों के उपयोग के लिए डॉ. जेम्स एल मैक्ग्रा को धन्यवाद देना चाहूंगा.

<ol>
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Methacrylic Anhydride [Internet] Available from: <a target="_blank" href="https://us.vwr.com/store/catalog/product.jsp?catalog_number=AAAL14357-18">https://us.vwr.com/store/catalog/product.jsp?catalog_number=AAAL14357-18</a> (2013)</li></ol>

लेखकों वे कोई प्रतिस्पर्धा वित्तीय हितों की है कि घोषित.

<table border="0" cellpadding="0" cellspacing="0" width="619">
	<tbody>
		<tr height="84">
			<td height="84" style="height:84px;width:216px;">3,6-Dioxa-1,8-octanedithiol</td>
			<td style="width:115px;">Tokyo Chemical Industry Co, LTD</td>
			<td style="width:119px;">D2649</td>
			<td style="width:167px;">CAS 14970-87-7</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Acetonitrile</td>
			<td style="width:115px;">J.T. Baker</td>
			<td style="width:119px;">UN1648</td>
			<td>CAS 75-05-8</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Amino Acids</td>
			<td style="width:115px;">AAPPTech</td>
			<td style="width:119px;">Glycine: AFG101</td>
			<td>CAS 29022-11-5</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;"> </td>
			<td style="width:115px;"> </td>
			<td style="width:119px;">Arginine: AFR105</td>
			<td>CAS 154445-77-9</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;"> </td>
			<td style="width:115px;"> </td>
			<td style="width:119px;">Asparagine: AFD105</td>
			<td>CAS 71989-14-5</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;"> </td>
			<td style="width:115px;"> </td>
			<td style="width:119px;">Serine: AFS105</td>
			<td>CAS 71989-33-8</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Anhydrous diethyl ether</td>
			<td style="width:115px;">Fisher Scientific</td>
			<td style="width:119px;">UN1155</td>
			<td>CAS 60-29-7</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Citric acid</td>
			<td style="width:115px;">Sigma Aldrich</td>
			<td style="width:119px;">C1857</td>
			<td>CAS 77-92-9</td>
		</tr>
		<tr height="84">
			<td height="84" style="height:84px;width:216px;">Deuterated chloroform</td>
			<td style="width:115px;">Cambridge Isotope Laboratories Inc.</td>
			<td style="width:119px;">DLM-7-100</td>
			<td>CAS 865-49-6</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Dichloromethane</td>
			<td style="width:115px;">Fisher Scientific</td>
			<td style="width:119px;">UN1593</td>
			<td>CAS 75-09-2</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Diisopropylethylamine</td>
			<td style="width:115px;">Alfa Aesar</td>
			<td style="width:119px;">A1181</td>
			<td>CAS 7087-68-5</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Dimethylformamide</td>
			<td style="width:115px;">Fisher Scientific</td>
			<td style="width:119px;">D119-4</td>
			<td>CAS 68-12-2</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">Fmoc-Gly-Wang resin</td>
			<td style="width:115px;">Peptides International</td>
			<td style="width:119px;">RGF-1301-PI</td>
			<td>100-200 mesh size</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Methacrylic anhydride</td>
			<td style="width:115px;">Alfa Aesar</td>
			<td style="width:119px;">L14357</td>
			<td>CAS 760-93-0</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">N-Methylpyrrolidone</td>
			<td style="width:115px;">VWR</td>
			<td style="width:119px;">BDH1141-4LG</td>
			<td>CAS 872-80-4</td>
		</tr>
		<tr height="84">
			<td height="84" style="height:84px;width:216px;">On-resin peptides</td>
			<td style="width:115px;">Synthesized in-house</td>
			<td style="width:119px;"> </td>
			<td style="width:167px;">On-resin peptides can also be purchased from Peptides International, GenScript, AAPPTec, etc.</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">O-Benzotriazole-N,N,N&#8217;,N&#8217;-tetramethyl-uronium-hexafluoro-phosphate</td>
			<td style="width:115px;">AnaSpec Inc</td>
			<td style="width:119px;">510/791-9560</td>
			<td>CAS 94790-37-1</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Peptide Calibration Standard</td>
			<td style="width:115px;">Care</td>
			<td style="width:119px;">206195</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Piperazine</td>
			<td style="width:115px;">Alfa Aesar</td>
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			<td height="42" style="height:42px;width:216px;">Poly(ethylene glycol) 10 kDa linear</td>
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</table>

microwave-assisted functionalization of poly(ethylene glycol) and on-resin peptides for use in chain polymerizations and hydrogel formation

पाली (इथाइलीन ग्लाइकॉल) (खूंटी) हाइड्रोजेल गठन में macromers का उपयोग करने के लिए मुख्य लाभ में से एक कृत्रिम चंचलता है. खूंटी आणविक भार और विन्यास की एक विशाल विविधता से आकर्षित करने की क्षमता (एआरएम संख्या, लंबाई हाथ, और शाखाओं में बंटी पैटर्न) हाइड्रोजेल संरचनाओं और यंग मापांक और जाल आकार सहित गुण, जिसके परिणामस्वरूप अधिक शोधकर्ताओं तंग नियंत्रण देता है. इस वीडियो पाली (इथाइलीन ग्लाइकॉल) dimethacrylate (PEGDM) में खूंटी व्यापारियों methacrylate के लिए एक तेजी से, कुशल, विलायक मुक्त, माइक्रोवेव की सहायता विधि समझाना होगा. इस कृत्रिम विधि दवा वितरण और पुनर्योजी चिकित्सा में अनुप्रयोगों के लिए बहुत जरूरी शुरू सामग्री प्रदान करता है. यह अभिकर्मकों और विलायकों की छोटी मात्रा का उपयोग काफी तेजी से और सरल, साथ ही और अधिक किफायती और पर्यावरण के अनुकूल है के रूप में प्रदर्शन किया विधि पारंपरिक methacrylation तरीकों से बेहतर है. हम यह भी राल methacr के लिए इस तकनीक का एक अनुकूलन प्रदर्शन करेंगेपेप्टाइड्स के ylamide functionalization. इस पर राल विधि पेप्टाइड्स के एन टर्मिनस से पहले राल से deprotection और दरार को methacrylamide समूहों के साथ क्रियाशील होने के लिए अनुमति देता है. प्रतिक्रियाशील ओर समूह (लाइसिन की जैसे प्राथमिक amine, सेरीन का प्राथमिक शराब, threonine के माध्यमिक एल्कोहल, और tyrosine के फिनोल) के साथ अमीनो एसिड functionalization रोकने, संरक्षित जबकि इस पेप्टाइड्स के एन टर्मिनी को methacrylamide समूहों के चुनिंदा इसके लिए अनुमति देता है कई स्थलों पर. यह आलेख विस्तार होगा आम विश्लेषणात्मक तरीकों; functionalizations की क्षमता का आकलन करने के लिए (प्रोटॉन परमाणु चुंबकीय अनुनाद स्पेक्ट्रोस्कोपी (एच एनएमआर) और मैट्रिक्स उड़ान मास स्पेक्ट्रोमेट्री की लेजर desorption ionization समय (MALDI-TOF) असिस्टेड). आम नुकसान और समस्या निवारण के तरीकों का सुझाव दिया है आगे धुन macromer कार्यक्षमता के लिए इस्तेमाल किया जा सकता है जो तकनीक और जिसके परिणामस्वरूप हाइड्रोजेल की इच्छा संशोधनों के रूप में संबोधित किया जाएगा भौतिक और रासायनिकगुण. विशेष ध्यान हाइड्रोजेल कठोरता और दवा रिहाई को नियंत्रित करने, जाल आकार को प्रभावित करने के लिए हाइड्रोजेल रचना को संशोधित करने के लिए भुगतान के साथ दवा वितरण और सेल सामग्री बातचीत के अध्ययन के लिए hydrogels के गठन के लिए संश्लेषित उत्पादों के उपयोग, प्रदर्शन किया जाएगा.

Watch this Scientific Journal Video about Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation at JoVE.com

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

इस वीडियो श्रृंखला polymerizations और हाइड्रोजेल संश्लेषण को सक्षम करने, पाली (इथाइलीन ग्लाइकॉल) methacrylate के लिए एक तेजी से, कुशल विधि समझाना होगा. यह इसी तरह, functionalization दक्षता का आकलन निवारण और उन्नत संशोधन के लिए सुझाव प्रदान करते हैं, और ठेठ हाइड्रोजेल लक्षण वर्णन तकनीक का प्रदर्शन करने के लिए पेप्टाइड्स, विस्तार आम विश्लेषणात्मक तरीकों में methacrylamide functionalities पेश करने के लिए प्रदर्शन करेंगे.

माइक्रोवेव की सहायता पाली के functionalization (इथाइलीन ग्लाइकॉल) और चैन Polymerizations और hydrogel संरचना में प्रयोग के लिए राल पेप्टाइड्स

One of the main benefits to using poly(ethylene glycol) (PEG) macromers in hydrogel formation is synthetic versatility. The ability to draw from a large ...

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Chemistry

Microwave-assisted Functionalization of Poly(ethylene glycol) and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes

माइक्रोवेव की सहायता intramolecular Functionalized Naphthalenes / Solvatochromic रंजक के संश्लेषण के लिए Dehydrogenative Diels Alder प्रतिक्रियाओं

Functionalized naphthalenes have applications in a variety of research fields ranging from the synthesis of natural or biologically active molecules to ...

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

लघु पेप्टाइड्स के आत्म विधानसभा के हिसाब से Biomolecular संरचनाओं के गठन

In nature, complex functional structures are formed by the self-assembly of biomolecules under mild conditions. Understanding the forces that control ...

Preparation of Silica Nanoparticles Through Microwave-assisted Acid-catalysis

माइक्रोवेव की सहायता एसिड कटैलिसीस के माध्यम से सिलिका नैनोकणों की तैयारी

Microwave-assisted synthetic techniques were used to quickly and reproducibly produce silica nanoparticle sols using an acid catalyst with nanoparticle ...

Ethylene Polymerizations Using Parallel Pressure Reactors and a Kinetic Analysis of Chain Transfer Polymerization

समानांतर दबाव रिएक्टरों और श्रृंखला स्थानांतरण polymerization के एक काइनेटिक विश्लेषण का उपयोग ईथीलीन polymerizations

We demonstrate a method for high-throughput catalyst screening using a parallel pressure reactor starting from the initial synthesis of a nickel ...

Synthesis, Characterization, and Functionalization of Hybrid Au/CdS and Au/ZnS Core/Shell Nanoparticles

संश्लेषण, लक्षण, और हाइब्रिड Au / सीडी और एयू / ZnS कोर / शैल नैनोकणों के functionalization

Plasmonic nanoparticles are an attractive material for light harvesting applications due to their easily modified surface, high surface area and large ...

One-pot Microwave-assisted Conversion of Anomeric Nitrate-esters to Trichloroacetimidates

एक पॉट माइक्रोवेव-Trichloroacetimidates करने के लिए Anomeric नाइट्रेट-एस्टर के रूपांतरण की सहायता

The goal of the following procedure is to provide a demonstration of the one-pot conversion of a 2-azido-1-nitrate-ester to a trichloroacetimidate ...

Surface Functionalization of Metal-Organic Frameworks for Improved Moisture Resistance

धातु की सतह Functionalization-कार्बनिक चौखटे में सुधार नमी प्रतिरोध के लिए

Metal-organic frameworks (MOFs) are a class of porous inorganic materials with promising properties in gas storage and separation, catalysis and sensing. ...

Reducing Willow Wood Fuel Emission by Low Temperature Microwave Assisted Hydrothermal Carbonization

कम तापमान माइक्रोवेव सहायता से विलो लकड़ी ईंधन उत्सर्जन को कम करने हाइड्रोथर्मल Carbonization

Biomass is a sustainable fuel, as its CO2 emissions are reintegrated in biomass growth. However, the inorganic precursors in the biomass cause a negative ...

Calcium Carbonate Formation in the Presence of Biopolymeric Additives

जैवबहुलक Additives की उपस्थिति में कैल्शियम कार्बोनेट गठन

Biomineralization is the formation of minerals in the presence of organic molecules, often related with functional and/or structural roles in living ...

Microwave-Assisted Preparation of 1-Aryl-1H-pyrazole-5-amines

Microwave-Assisted Preparation of 1-Aryl-1H-pyrazole-5-amines

माइक्रोवेव-सहायक 1-एरिल-1एच-पायराज़ोल-5-एमिन्स की तैयारी

A synthetic process for the preparation of a variety of 1-aryl-1H-pyrazole-5-amines was developed. The microwave-mediated nature of this method makes it ...