Name: reproducable paraplegia by thoracic aortic occlusion in a murine model of spinal cord ischemia-reperfusion
Uploaded: 2014-03-03T21:55:00
Description: Watch this Scientific Journal Video about Reproducable Paraplegia by Thoracic Aortic Occlusion in a Murine Model of Spinal Cord Ischemia-reperfusion at JoVE.com

我们要感谢的胸外科基础研究和教育项目的资金支持。

1。术前准备和麻醉<ol><li>一定要在整个过程中观察无菌技术。奠定了所有仪器。 </li><li>打开前麻醉诱导温度控制床，以便它可以加热至适当的温度（36.5℃）。功率激光多普勒血流灌注监视器上，以便它可以诱导过程中启动。 </li><li>将鼠标放置在感应室。 <ol><li>仔细监视鼠标的感应过程中的呼吸频率。 </li><li>只要呼吸速率已目视减缓，从感应腔移开鼠标。 </li><li>执行脚趾捏来评估麻醉剂是否?...

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截瘫继发于脊髓缺血再灌注损伤是知之甚少病状9的复合物的结果。虽然这是胸腹主动脉瘤手术后最常见的，各种其他辱骂如主动脉夹层，创伤，栓塞现象，血管炎和系统性低血压10可导致截瘫。为了获得进一步的了解这个伤害，并为未来的目标，以消除这种损伤，动物模型已经成为必然。 患者患有这种并发症展览要么是立即或延迟瘫痪。这种模式和其他人充?...

下肢瘫痪的不断复杂化胸腹干预。这次受伤，被称为脊髓缺血再灌注损伤（SCIR），导致瘫痪的高危患者1的高达20％。外科手术辅助系统，例如左心脏搭桥，腰椎cerbrospinal流体水渠，低温停循环和肋间动脉再植减少这种并发症2的发病率，但是，太多的患者继续受到影响。 在临床上，脊髓缺血再灌注损伤被看作是立即或延迟瘫痪以下干预3。然而，我们的这种损伤的认识已经扼杀了机械缺乏细节。其结果是，几个选项可用来衰减一旦发生损伤。 因此，我们已委托一个小动物，鼠，模型脊髓缺血和再灌注损伤更好地表征其发病机制。大多数研究迄今已经使用更大的动物模型来描述这种损伤，即大鼠4，兔5，和猪6款车型。然而，这些是由它们的成本，复杂性，可变的再现性，并且，最重要的是，缺乏对遗传操作可行技术的限制。最可靠的这些公布的动物模型包括兔腹主动脉的肾下交叉夹紧。然而，人类的脊髓前神经元最经常得到他们的血管供应的更近端分支7。在这些模型的脊髓可变血管解剖增加了难度在过渡其结果投入临床使用。 这个手稿提出了一个模型，立即或延迟截瘫以下胸主动脉阻塞是临床相关且易于使用。通过曝光迷你sternoto主动脉弓我是创伤小，能引起高度重复性的结果，最少的发病率和死亡率。虽然这种模式在并非没有挑战和技术的细微差别，这些都可以通过仔细解剖和组织处理克服生产的后肢瘫痪了一个模型，可以很容易地实现。

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			<td height="21" style="height:21px;width:216px;">VMS Anesthesia Machine</td>
			<td style="width:247px;">MDS Matrx</td>
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			<td style="width:167px;"></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Isoflurane</td>
			<td style="width:247px;">Vet One</td>
			<td style="width:119px;">13985-528-60</td>
			<td>2.0% through nose cone&#160;</td>
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			<td height="21" style="height:21px;width:216px;">Induction Chamber</td>
			<td style="width:247px;">Vet Equip</td>
			<td>941444</td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Heating Bed</td>
			<td style="width:247px;">Vestavia Scientific</td>
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			<td></td>
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			<td height="21" style="height:21px;width:216px;">Laser Doppler Monitor</td>
			<td style="width:247px;">Moor Instruments</td>
			<td style="width:119px;">VMS-LDF1</td>
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			<td height="21" style="height:21px;width:216px;">5-0 Suture, Polyester</td>
			<td style="width:247px;">Surgidac</td>
			<td style="width:119px;">VD-551</td>
			<td>Taper Needel</td>
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			<td height="25" style="height:25px;width:216px;">Microdissecting Clips</td>
			<td style="width:247px;">Biomedical Research Insturments</td>
			<td style="width:119px;">14-1030, 14-1060</td>
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			<td height="25" style="height:25px;width:216px;">Surgical Instruments</td>
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			<td>Forceps, needle holder</td>
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reproducable paraplegia by thoracic aortic occlusion in a murine model of spinal cord ischemia-reperfusion

背景 下肢瘫痪的不断复杂主动脉干预。的基本病理认识的缺乏阻碍了进步，以减少发生这种损伤。目前的模型表明重现性下肢瘫痪下面的胸主动脉闭塞。 方法 成年雄性C57BL6小鼠用异氟烷麻醉。通过cervicosternal切口主动脉被曝光。胸降主动脉和左锁骨下动脉被认定不进入胸膜腔。这些动脉的骨架，其次是立即关闭（深）或闭塞4分钟（中度缺血）或8分（长期缺血）。在胸骨和皮肤被关闭，鼠标被转移到气候变暖床上进行恢复。以下恢复，在12小时的时间间隔获得的功能分析，直到48小时。 业绩 小鼠接受假手术均未见观察后肢赤字。老鼠受到中度缺血4分钟有最小功能缺损在12小时之后的进展在48小时才能完成瘫痪。小鼠长期遭受缺血产生了立竿见影的麻痹，没有观察后肢运动在术后的任何一点。没有观察到术中或术后死亡率。 结论 重现性下肢瘫痪是否立即或延迟可在小鼠模型来实现。此外，通过采用胸骨正中切开，并小心剥离，高生存率和重现性得以实现。

小鼠进行假手术（3例）或主动脉闭塞4（N = 3）到8分钟（N = 3）。术后小鼠由巴索鼠标分数（ 图1）进行分级。小鼠接受假手术有没有明显的功能障碍在任何时候术后。老鼠受到中度缺血（4分）有接近正常的后肢功能在12小时渐进功能下降由48小时来完成瘫痪。小鼠在长时间缺血组（8分钟）有完全麻痹手术后没有任何可收回功能（图2）。 <p class="jove_content" fo:keep-together.w...

Watch this Scientific Journal Video about Reproducable Paraplegia by Thoracic Aortic Occlusion in a Murine Model of Spinal Cord Ischemia-reperfusion at JoVE.com

Reproducable Paraplegia by Thoracic Aortic Occlusion in a Murine Model of Spinal Cord Ischemia-reperfusion

可重放截瘫胸主动脉阻断脊髓缺血再灌注的小鼠模型

脊髓缺血再灌注损伤的机理认识的缺乏阻碍了进一步的附属物，以防止截瘫以下高风险的主动脉操作。因此，动物模型的开发是必不可少的。这个手稿展示了可重复的下肢瘫痪继小鼠模型主动脉闭塞。

Background
Lower extremity paralysis continues to complicate aortic interventions. The lack of understanding of the underlying pathology has hindered ...

Research

JoVE Journal

Medicine

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

在大鼠脊髓损伤后的急性和慢性触觉测试

Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

科研

医学

A Contusion Model of Severe Spinal Cord Injury in Rats

挫伤模型脊髓严重损伤的保护作用

The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is ...

A Murine Model of Cervical Spinal Cord Injury to Study Post-lesional Respiratory Neuroplasticity

颈脊髓损伤的小鼠模型研究后皮损呼吸神经可塑性

A cervical spinal cord injury induces permanent paralysis, and often leads to respiratory distress. To date, no efficient therapeutics have been developed ...

A Murine Model of Myocardial Ischemia-reperfusion Injury through Ligation of the Left Anterior Descending Artery

心肌缺血再灌注损伤的通过左前降支结扎小鼠模型

Acute or chronic myocardial infarction (MI) are cardiovascular events resulting in high morbidity and mortality. Establishing the pathological mechanisms ...

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

无线电遥测系统，以监测大鼠脊髓横断和胚胎神经干细胞移植在心血管功能

High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

A Neonatal Mouse Spinal Cord Compression Injury Model

新生儿小鼠脊髓压迫损伤模型

Spinal cord injury (SCI) typically causes devastating neurological deficits, particularly through damage to fibers descending from the brain to the spinal ...

Murine Model of Intestinal Ischemia-reperfusion Injury

肠缺血再灌注损伤的小鼠模型

Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, ...

Reproducible Motor Deficit Following Aortic Occlusion in a Rat Model Of Spinal Cord Ischemia

主动脉阻塞后脊髓缺血大鼠模型中的可再生电机缺陷

Spinal cord ischemia is a fatal complication following thoracoabdominal aortic aneurysm surgery. Researchers can investigate the strategies for preventing ...

Two-vessel Occlusion Mouse Model of Cerebral Ischemia-reperfusion

脑缺血再灌注的双血管闭塞小鼠模型

In this study, a middle cerebral artery (MCA) occlusion mouse model is employed to study cerebral ischemia-reperfusion. A reproducible and reliable mouse ...

Evaluation of Coronary Flow Reserve After Myocardial Ischemia Reperfusion in Rats

大鼠心肌缺血再灌注后冠状动脉流动储备评价

Coronary artery disease is the leading cause of death worldwide. After an acute myocardial infarction, early and successful myocardial intervention via ...