Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/50921-v
October 30th, 2013
Chapters
0:05
Title
1:19
Purification of 6xHIS-tagged Proteins Expressed in Budding Yeast Cells Under Native Conditions
3:01
Homogenization of Yeast Cells and Extraction of Proteins
4:06
Batch Purification of Proteins from Yeast Cell Homogenates
6:23
Representative Yeast Extraction and Purification Results
8:57
Conclusion
高品質の酵母細胞抽出物の調製は、個々のタンパク質または全体プロテオームの解析に必要な最初のステップである。ここでは、タンパク質機能、相互作用、および翻訳後修飾を維持するために最適化された出芽酵母細胞のための、高速で効率的、かつ信頼性の高い均質化プロトコルが記載されている。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved