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DOI :
10.3791/50930-v
•
12:04 min
April 2nd, 2014
Chapters
0:05
Title
1:18
Identifying TALEN Target Sites
2:30
Embryo Isolation
4:23
Embryo Microinjection
6:33
Embryo Transfer
9:15
Identifying Founders with Targeted Mutations
11:21
Conclusion
设计师核酸酶如锌指核酸酶(ZFN)和转录活化因子样效应核酸酶(TALENS)可用于通过触发两个非同源末端连接(NHEJ)和同源重组(HR)途径来修改小鼠早期胚胎的基因组。这些进展使快速生成小鼠的精确的遗传修饰。
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