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DOI :
10.3791/50948-v
•
11:33 min
December 30th, 2013
Chapters
0:05
Title
1:55
Gel Electrophoresis with Stain-free Gels and Ge Imaging
3:29
Protein Transfer with Trans-blot Turbo System and Antibody Incubation
6:32
Imaging and Calculating the Total Protein Normalization: Image Lab
8:53
Total Protein Normalization Results for MCM-7 in Irradiated Lymphoblastoid Cells
10:43
Conclusion
V3 工作流是使用无污渍凝胶的西方污点程序。无污渍技术使研究人员能够可视化蛋白质分离质量,验证转移效率,最重要的是,使用总蛋白质定量作为可靠的加载控制来验证兴趣蛋白的变化。
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