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DOI :
10.3791/50948-v
•
11:33 min
December 30th, 2013
Chapters
0:05
Title
1:55
Gel Electrophoresis with Stain-free Gels and Ge Imaging
3:29
Protein Transfer with Trans-blot Turbo System and Antibody Incubation
6:32
Imaging and Calculating the Total Protein Normalization: Image Lab
8:53
Total Protein Normalization Results for MCM-7 in Irradiated Lymphoblastoid Cells
10:43
Conclusion
V3ワークフローは、染色フリーゲルを使用したウェスタンブロット手順です。この染色フリー技術により、研究者はタンパク質分離の質を可視化し、転写効率を検証し、最も重要なのは、信頼性の高いローディングコントロールとしてタンパク質の総定量を使用して目的のタンパク質の変化を検証することが可能です。
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