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DOI :
10.3791/50948-v
•
11:33 min
December 30th, 2013
Chapters
0:05
Title
1:55
Gel Electrophoresis with Stain-free Gels and Ge Imaging
3:29
Protein Transfer with Trans-blot Turbo System and Antibody Incubation
6:32
Imaging and Calculating the Total Protein Normalization: Image Lab
8:53
Total Protein Normalization Results for MCM-7 in Irradiated Lymphoblastoid Cells
10:43
Conclusion
V3 워크플로우는 얼룩이 없는 젤을 사용한 웨스턴 블롯 시술입니다. 얼룩없는 기술은 연구원이 단백질 분리 품질을 시각화하고, 전달 효율을 검증하며, 가장 중요한 것은 신뢰할 수있는 적단백질 정량화를 사용하여 관심있는 단백질의 변화를 검증 할 수 있게합니다.
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