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DOI :
10.3791/50951-v
September 19th, 2013
Chapters
0:05
Title
1:11
Preparation of the Tissue
2:45
Typical Enzymatic Tissue Digestion to Obtain a Single Cell Suspension
4:35
Enrichment of Low Density Fraction (LDF) of Cells Using Percoll Density Separation
6:00
Results: Representative Myeloid DC and TEC Populations Isolated from the Human Thymus
8:06
Conclusion
이 프로토콜은 항원 밀도 단일 세포 현탁액을 원심 분리하고 그 세포 집단의 마지막 자석 및 / 또는 FACS 정렬 뒤에 조직의 소화 효소의 여러 단계를 통해 인간 흉선에서 제시 세포 분리하는 방법을 자세히 설명.
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