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DOI :
10.3791/50961-v
•
12:33 min
November 15th, 2013
Chapters
0:05
Title
2:18
Assay Set-up
4:00
Incubation of Assay Plates
4:56
Fluorescent Measurements on a Microplate Fluorometer
7:03
Results: Analysis of Extracellular Enzyme Activity (EEA)
11:05
Conclusion
5:43
Data Analysis
测量土壤胞外酶活性的潜在利率,绑定到一个荧光染料的合成底物加入到土壤样品。酶的活性被测量为荧光染料从基板释放的酶催化反应,其中更高的荧光指示多底物降解。
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