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DOI :
10.3791/50964-v
•
12:30 min
November 22nd, 2013
Chapters
0:05
Title
1:38
Preparation of L. pneumophila for Infection
3:04
Preparation and Infection of G. mellonella
5:39
Analysis of Larval Mortality
6:17
Extraction and Processing of Hemolymph
8:57
Quantifying the Bacterial CFU
9:37
Virulence of WT and Dot/Icm-deficient L. pneumophila
12:07
Conclusion
蜡蛾蜡螟的幼虫最近被确立为一种体内模型来研究嗜肺军团菌感染。在这里,我们展示了基本技术, 军团菌的发病特点的幼虫,包括接种,细菌毒力和复制的测量和提取被感染的血细胞和分析。
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