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DOI :
10.3791/50981-v
April 1st, 2014
Chapters
0:05
Title
1:01
Preparing Placenta for Dissection
4:49
Homogenizing Placental Villous Cells and Isolating Lipid Droplets by Ultracentrifugation
7:43
Results: Isolation of Lipid Droplets from Placental Villous Cells
9:16
Conclusion
两种技术从1分离细胞的脂滴),酵母细胞和2)人类胎盘介绍。这两个程序的核心是密度梯度离心法,在这里可以用肉眼很容易地可视化含有液滴产生的浮层,提取,并通过Western印迹分析纯度量化。
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