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DOI :
10.3791/50989-v
•
11:32 min
November 12th, 2013
Chapters
0:05
Title
1:19
Dissection and Dissociation of Rat RMS Neuroblasts
4:30
Nucleofection
6:43
Embedding
8:50
Results: Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
11:09
Conclusion
成神经细胞迁移是在出生后的神经发生了关键性的一步。在这里描述的协议可以被用于研究神经细胞迁移的候选调节器通过使用DNA /小发夹RNA(shRNA的)核转染,并与成神经细胞从啮齿动物出生后的头端迁移流中分离出来的三维迁移实验中的作用。
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