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DOI :
10.3791/50989-v
•
11:32 min
November 12th, 2013
Chapters
0:05
Title
1:19
Dissection and Dissociation of Rat RMS Neuroblasts
4:30
Nucleofection
6:43
Embedding
8:50
Results: Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
11:09
Conclusion
神経芽細胞の遊走は、出生後の神経新生に重要なステップです。プロトコルは、ここに記載DNA /低分子ヘアピンRNA(shRNAは)ヌクレオおよびげっ歯類生後吻側遊走性流から単離された神経芽細胞を用いた3次元遊走アッセイを用いることにより、神経芽細胞遊走の候補調節因子の役割を調査するために使用することができる。
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