Name: diagnosis of neoplasia in barrett&#8217;s esophagus using vital-dye enhanced fluorescence imaging
Uploaded: 2014-05-11T16:00:00
Description: Watch this Scientific Journal Video about Diagnosis of Neoplasia in BarrettÃ¢â‚¬â„¢s Esophagus using Vital-dye Enhanced Fluorescence Imaging at JoVE.com

这项工作是由美国国家癌症研究所的卫生补助R01 CA140257-01的国家研究所的支持。

<html> 注：签署了知情同意的患者。此外，这项研究已在符合人类福祉的所有机构，国家和国际准则进行的。 1，准备电脑<ol><li>打开笔记本电脑，并连接USB从DVI2USB采集卡。 </li></ol> 2，准备监视器<ol><li>连接DVI缆线，以监测和画中画，以允许内窥镜医生从这些画面观看视频，而不是计算机。 </li><li>确保站在显示器上，并设置为DVI。 </li><li>连接...

<ol>
	<li>Modiano, N., Gerson, L. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Barrett's+Esophagus:+Incidence,+etiology,+pathophysiology,+prevention+and+treatment.">Barrett's Esophagus: Incidence, etiology, pathophysiology, prevention and treatment.</a> Therapeutics and Clinical Risk Management. 3, 1035-1145 (2007).</li><li>Brown, L. M., Devesa, S. S., Chow, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Incidence+of+Adenocarcinoma+of+the+esophagus+among+white+Americans+by+sex,+stage,+and+age.">Incidence of Adenocarcinoma of the esophagus among white Americans by sex, stage, and age.</a> Journal of the National Cancer Institute. 100, 1184-1187 (2008).</li><li>Siegel, R., Naishadham, D., Ahmedin, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cancer+Statistics,+2012.">Cancer Statistics, 2012.</a> A Cancer Journal for Clinicians. 62, 10-29 (2012).</li><li>Vieth, M., Ell, C., Gossner, L., May, A., Stolte, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histological+analysis+of+endoscopic+resection+specimens+from+326+patients+with+Barrett's+esophagus+and+early+neoplasia.">Histological analysis of endoscopic resection specimens from 326 patients with Barrett's esophagus and early neoplasia.</a> Endoscopy. 36, 776-781 (2004).</li><li>Pohl, H., Rosch, T., Vieth, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Miniprobe+confocal+laser+microscopy+for+the+detection+of+invisible+neoplasia+in+patients+with+Barrett's+oesophagus.">Miniprobe confocal laser microscopy for the detection of invisible neoplasia in patients with Barrett's oesophagus.</a> Gut. 57, 1648-1653 (2008).</li><li>Thekkek, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modular+video+endoscopy+for+in+vivo+cross-polarized+and+vital-dye+fluorescence+imaging+of+Barrett's-associated+neoplasia.">Modular video endoscopy for in vivo cross-polarized and vital-dye fluorescence imaging of Barrett's-associated neoplasia.</a> Journal of Biomedical Optics. 18, 026007 (2013).</li><li>Roblyer, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multispectral+optical+imaging+device+for+in+vivo+detection+of+oral+neoplasia.">Multispectral optical imaging device for in vivo detection of oral neoplasia.</a> Journal of Biomedical Optics. 13, 024019 (2008).</li><li>Egger, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biopsy+surveillance+is+still+necessary+in+patients+with+Barrett's+oesophagus+despite+new+endoscopic+imaging+techniques.">Biopsy surveillance is still necessary in patients with Barrett's oesophagus despite new endoscopic imaging techniques.</a> Gut. 52, 18-23 (2003).</li><li>Thekkek, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vital-dye+enhanced+fluorescence+imaging+of+GI+mucosa:+metaplasia,+neoplasia,+inflammation.">Vital-dye enhanced fluorescence imaging of GI mucosa: metaplasia, neoplasia, inflammation.</a> Gastrointestinal Endoscopy. 75, 877-887 (2012).</li><li>Muldoon, T. J., Anandasabapathy, S., Maru, D., Richards-Kortum, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-resolution+imaging+in+Barrett's+esophagus:+a+novel,+low-cost+endoscopic+microscope.">High-resolution imaging in Barrett's esophagus: a novel, low-cost endoscopic microscope.</a> Gastrointestinal Endoscopy. 68, 737-744 (2008).</li></ol>

与标准的内镜检查，在BE肿瘤常常被漏诊8，因为良性上皮化生可以从高度异型增生和腺癌难以区别。作为一种工具，将更好地使胃肠病来解决这个问题目前无法避免的错误，重要的染料增强荧光成像突出了组织的腺体形态从而提供了一个独特的功能来区分组织类型。此外，通过提供的视图长达2.5厘米的领域，VFI使内镜医师能够有效地和有系统地平移，在整个食管远端，使可疑病变更为突?...

在过去的四十年，食管腺癌（EAC）的发病率显著增加1,2;但由于后期诊断，五年生存率小于20％3。在BE内镜检查的现行标准，前体选管会，是白光内镜与段的随机四quandrant镊子&#39;活组织切片检查。不幸的是，这种技术常常射门瘤形成，它可以是平的，细微且难以区分的标准白光成像4。虽然已经在使用激光共聚焦显微镜突出体内细胞功能的成功，病变仍可由于视图的5场减少错过。有一个“桥梁”技术，它可以突出区域进行进一步的共聚焦显微成像将是显着的价值。 因此，增强红旗成像方式，提高到精华素的能力T和活检早期肿瘤在BE将有助于检测选管会在早期，可医治的阶段，并可能导致更有效的治疗，并随后提高生存率。 VFI是一种新型的技术，它结合了高解析度成像上皮外源外用荧光造影，原黄素，突出腺体形态并划定肿瘤（高度异型增生和癌症）在食管远端在改善体内诊断6的希望。经原黄素，该申请后不久，浓缩细胞核内的激发下，荧光图像提供50至100微米的空间分辨率和视高达2.5厘米的字段，允许内镜形象化腺形态。作为一个结果，这个方法使肠胃病学区分经典巴雷特化生，其中有连续的，均匀间隔的腺体和整体均匀的形态，从可以与瘤形成，其具有obliteration的腺体结构。在这里，我们描述了这种新技术的协议与多光谱内窥镜，并提供有代表性的结果证明该设备的描述中良性上皮化生的形态转变为高度异型增生和癌症的效用。

<table border="0" cellpadding="0" cellspacing="0" width="650">
	<tbody>
		<tr>
			<td>Filter*</td>
			<td>Schott North America, Inc., Duryea, Pennsylvania</td>
			<td>Not Applicable</td>
			<td>495-nm long-pass filter</td>
		</tr>
		<tr>
			<td>Halo Cap &#8211; Medium*</td>
			<td>Barrx Medical</td>
			<td>CP-002A</td>
			<td></td>
		</tr>
		<tr>
			<td>Processor*</td>
			<td>Pentax</td>
			<td>EPK-i</td>
			<td></td>
		</tr>
		<tr>
			<td>Multispectral Digital Microscope**</td>
			<td>Not Applicable</td>
			<td>Not Applicable</td>
			<td></td>
		</tr>
		<tr>
			<td>Kimwipes</td>
			<td>Kimberly-Clark</td>
			<td>KimTech Science</td>
			<td>S-8115</td>
		</tr>
		<tr>
			<td>Cidex</td>
			<td>Advanced Sterilization Products&#160;</td>
			<td>CIDEX OPA Solution</td>
			<td></td>
		</tr>
		<tr>
			<td>Proflavine hemisulfate (0.01% w/v)</td>
			<td></td>
			<td></td>
			<td>FDA (IND 102,217)</td>
		</tr>
		<tr>
			<td>Laser Diode*</td>
			<td>Nichia Corporation</td>
			<td>Not Applicable</td>
			<td>455-nm</td>
		</tr>
		<tr>
			<td>Image Capture*</td>
			<td>Labview</td>
			<td>Not Applicable</td>
			<td></td>
		</tr>
		<tr>
			<td>Spray Catheter</td>
			<td>Olympus</td>
			<td>Not Applicable</td>
			<td></td>
		</tr>
		<tr>
			<td></td>
			<td></td>
			<td></td>
			<td>*Equipment specifics within Reference 6. **Equipment specifics within Reference 7</td>
		</tr>
	</tbody>
</table>

diagnosis of neoplasia in barrett&#8217;s esophagus using vital-dye enhanced fluorescence imaging

从肿瘤在体内分化良性上皮化生Barrett食管（BE）的能力仍然是困难的，因为这两种组织类型可以是平的，并没有什么区别与单纯的白色光成像。其结果是，一个模式，突出腺架构将是有益的在食管远端良性上皮肿瘤鉴别。 VFI是一个使用外源性外用荧光造影剂来界定高档异型增生和癌症良性上皮细胞新技术。具体地，荧光图像提供50至100微米的空间分辨率和视高达2.5厘米的字段，允许内镜形象化腺形态。一旦激发，经典巴雷特化生表现为连续，均匀间隔的腺体和整体均匀的形态;与此相反，肿瘤组织出现拥挤腺体框架完全闭塞。在这里，我们提供的概述仪器仪表和枚举这种新技术的协议。虽然VFI提供了一次胃肠病与可疑组织的腺体架构，细胞不典型增生不能用这种方式解决。因此，人们不能形态学通过该成像方式区分巴雷特化生从与低度不典型增生。通过权衡在与视图更大字段分辨率的降低，该成像系统可用于在至少一个红色标记成像器件对目标和活检可疑病灶;然而，如果精度的措施是令人鼓舞的，VFI可能成为肿瘤的诊断（定义为高档异型增生或癌症）在食管远端的标准成像技术。

图1B描绘了经典的Barrett食管，没有发育不良正常鳞状上皮所包围的边框。与较平坦的鳞状组织，其圆周分布，并用蓝色箭头表示开始，无光泽荧光的均匀区域的存在与无腺体结构。绿色箭头表示周围的鳞状上皮组织的圆形绿线。本大纲是神器从内窥镜的上限所致。移动到位于中心位置的Barrett的组织，腺体结构可以被定义为周围较暗的管腔绿色荧光。虽然一些腺被拉长，有相邻的腺体...

Watch this Scientific Journal Video about Diagnosis of Neoplasia in BarrettÃ¢â‚¬â„¢s Esophagus using Vital-dye Enhanced Fluorescence Imaging at JoVE.com

Diagnosis of Neoplasia in BarrettÃƒÂ¢Ã¢â€šÂ¬Ã¢â€žÂ¢s Esophagus using Vital-dye Enhanced Fluorescence Imaging

用生命染增强荧光成像瘤在Barrett食管诊断

重要的染料增强荧光成像（VFI）是生物体内的技术了一种新的，结合高分辨率成像上皮外源外用荧光对比，从而突出腺体形态及食管远端划定瘤（高档异型增生和癌症）。

The ability to differentiate benign metaplasia in Barrett&#8217;s Esophagus (BE) from neoplasia in vivo remains difficult as both tissue types can be flat ...

Research

JoVE Journal

Bioengineering

Diagnosis of Neoplasia in Barrett&#8217;s Esophagus using Vital-dye Enhanced Fluorescence Imaging

Three-dimensional Optical-resolution Photoacoustic Microscopy

三维光学分辨率的光声显微镜

Optical microscopy, providing valuable insights at the cellular and organelle levels, has been widely recognized as an enabling biomedical technology. As ...

科研

生物工程

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

在活细胞中的荧光寿命成像分子转子

Diffusion is often an important rate-determining step in chemical reactions or biological processes and plays a role in a wide range of intracellular ...

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip

时间推移荧光成像拟南芥根系生长的根环境的快速操作使用RootChip

The root functions as the physical anchor of the plant and is the organ responsible for uptake of water and mineral nutrients such as nitrogen, ...

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

受体动力学通过单分子荧光显微镜的高分辨率时空分析

Single-molecule microscopy is emerging as a powerful approach to analyze the behavior of signaling molecules, in particular concerning those aspect (e.g., ...

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3d轨道跟踪的改进双光子显微镜之应用：细胞内的囊泡的跟踪

The objective of this video protocol is to discuss how to perform and analyze a three-dimensional fluorescent orbital particle tracking experiment using a ...

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

从快速荧光成像在分子扩散法对活细胞的膜在商业显微镜

It has become increasingly evident that the spatial distribution and the motion of membrane components like lipids and proteins are key factors in the ...

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

生物膜荧光探针力：受体 - 配体动力学并行和定量结合诱导细胞内信号作用于单细胞

Membrane receptor-ligand interactions mediate many cellular functions. Binding kinetics and downstream signaling triggered by these molecular interactions ...

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip

使用多通道双电场微流控芯片肺癌细胞Electrotaxis研究

The behavior of directional cell migration under a direct current electric-field (dcEF) is referred to as electrotaxis. The significant role of ...

Conducting Multiple Imaging Modes with One Fluorescence Microscope

使用一个荧光显微镜进行多种成像模式

Fluorescence microscopy is a powerful tool to detect biological molecules in situ and monitor their dynamics and interactions in real-time. In addition to ...

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

利用先进的纺额圆盘全内反射荧光显微镜观察细胞粘附形成

In living cells, processes such as adhesion formation involve extensive structural changes in the plasma membrane and the cell interior. In order to ...