Name: assessing myogenic response and vasoactivity in resistance mesenteric arteries using pressure myography
Uploaded: 2015-07-06T13:00:00
Description: Watch this Scientific Journal Video about Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography at JoVE.com

Sandeep Khurana wordt ondersteund door NIH (K08DKO81479). Vikrant Rachakonda wordt ondersteund door (T32DK067872).

De hier getoonde voorbeelden zijn van experimenten van IACUC goedgekeurd Georgia Regents University - Protocol nr: # 2011-0408 1. Bereiding van reagentia <ol><li> Bereid dissectie oplossing stock: Voor 500 ml van de dissectie-oplossing (5x), ontbinden 21.18 g NaCl, 0,875 g KCI, 0,739 g MgSO4, 1,049 g MOPS en 0,019 g EDTA in 450 ml Milli-Q water. Pas de pH op 7,3-7,4 met behulp van 1 N NaOH. Maak het volume tot 500 ml met Milli-Q water. De oplossing kan worden bewaard tot 7-10 da...

<ol>
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J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+myogenic+response:+established+facts+and+attractive+hypotheses.">The myogenic response: established facts and attractive hypotheses.</a> Clin Sci (Lond). 96, 313-326 (1999).</li><li>Bridges, L. E., Williams, C. L., Pointer, M. A., Awumey, E. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mesenteric+artery+contraction+and+relaxation+studies+using+automated+wire+myography.">Mesenteric artery contraction and relaxation studies using automated wire myography.</a> J Vis Exp. , (2011).</li><li>Buus, N. H., Vanbavel, E., Mulvany, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differences+in+Sensitivity+of+Rat+Mesenteric+Small+Arteries+to+Agonists+When+Studied+as+Ring+Preparations+or+as+Cannulated+Preparations.">Differences in Sensitivity of Rat Mesenteric Small Arteries to Agonists When Studied as Ring Preparations or as Cannulated Preparations.</a> British Journal of Pharmacology. 112, 579-587 (1994).</li><li>Falloon, B. J., Stephens, N., Tulip, J. R., Heagerty, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+small+artery+sensitivity+and+morphology+in+pressurized+and+wire-mounted+preparations.">Comparison of small artery sensitivity and morphology in pressurized and wire-mounted preparations.</a> Am J Physiol. 268, H670-H678 (1995).</li><li>Schubert, R., Wesselman, J. P. M., Nilsson, H., Mulvany, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noradrenaline-induced+depolarization+is+smaller+in+isobaric+compared+to+isometric+preparations+of+rat+mesenteric+small+arteries.">Noradrenaline-induced depolarization is smaller in isobaric compared to isometric preparations of rat mesenteric small arteries.</a> Pflugers Archiv-European Journal of Physiology. 431, 794-796 (1996).</li><li>Khurana, S., Raina, H., Pappas, V., Raufman, J. P., Pallone, T. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+deoxycholylglycine,+a+conjugated+secondary+bile+acid,+on+myogenic+tone+and+agonist-induced+contraction+in+rat+resistance+arteries.">Effects of deoxycholylglycine, a conjugated secondary bile acid, on myogenic tone and agonist-induced contraction in rat resistance arteries.</a> PLoS One. 7, e32006 (2012).</li><li>Bayliss, W. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=On+the+local+reactions+of+the+arterial+wall+to+changes+of+internal+pressure.">On the local reactions of the arterial wall to changes of internal pressure.</a> J Physiol. 28, 220-231 (1902).</li><li>Hughes, J. M., Bund, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Arterial+myogenic+properties+of+the+spontaneously+hypertensive+rat.">Arterial myogenic properties of the spontaneously hypertensive rat.</a> Exp Physiol. 87, 527-534 (2002).</li><li>Mulvany, M. J., Halpern, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanical+properties+of+vascular+smooth+muscle+cells+in+situ.">Mechanical properties of vascular smooth muscle cells in situ.</a> Nature. 260, 617-619 (1976).</li><li>Raina, H., Zacharia, J., Li, M., Wier, W. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Activation+by+Ca2+/calmodulin+of+an+exogenous+myosin+light+chain+kinase+in+mouse+arteries.">Activation by Ca2+/calmodulin of an exogenous myosin light chain kinase in mouse arteries.</a> J Physiol. 587, 2599-2612 (2009).</li><li>Fenger-Gron, J., Mulvany, M. J., Christensen, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mesenteric+blood+pressure+profile+of+conscious,+freely+moving+rats.">Mesenteric blood pressure profile of conscious, freely moving rats.</a> J Physiol. 488 (Pt 3), 753-760 (1995).</li><li>Davis, M. J., Hill, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Signaling+mechanisms+underlying+the+vascular+myogenic+response).">Signaling mechanisms underlying the vascular myogenic response).</a> Physiol Rev. 79, 387-423 (1999).</li><li>Osmond, J. M., Mintz, J. D., Dalton, B., Stepp, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Obesity+increases+blood+pressure,+cerebral+vascular+remodeling,+and+severity+of+stroke+in+the+Zucker+rat.">Obesity increases blood pressure, cerebral vascular remodeling, and severity of stroke in the Zucker rat.</a> Hypertension. 53, 381-386 (2009).</li><li>Ge, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endogenously+produced+20-HETE+modulates+myogenic+and+TGF+response+in+microperfused+afferent+arterioles.">Endogenously produced 20-HETE modulates myogenic and TGF response in microperfused afferent arterioles.</a> Prostaglandins Other Lipid Mediat. 102-103, 42-48 (2013).</li><li>Ryan, M. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Placental+ischemia+impairs+middle+cerebral+artery+myogenic+responses+in+the+pregnant+rat.">Placental ischemia impairs middle cerebral artery myogenic responses in the pregnant rat.</a> Hypertension. 58, 1126-1131 (2011).</li><li>Bagi, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Type+2+diabetic+mice+have+increased+arteriolar+tone+and+blood+pressure:+enhanced+release+of+COX-2-derived+constrictor+prostaglandins.">Type 2 diabetic mice have increased arteriolar tone and blood pressure: enhanced release of COX-2-derived constrictor prostaglandins.</a> Arterioscler Thromb Vasc Biol. 25, 1610-1616 (2005).</li></ol>

Kritische stappen, het oplossen van problemen en wijzigingen In een typische bereiding isobare vat, wordt de slagader op druk 70 mmHg tussen twee glazen canules geperfuseerd met warm (37 ° C) PSS. Na 30-45 min, bloedvaten ontwikkelt MT, gekenmerkt door spontane verlaging in diameter die stabiliseert in 20-30 min. De weerstand slagaders van verschillende vasculaire bedden ontwikkelen variabele MT. Bijvoorbeeld weerstand rat mesenteriale slagaders ontwikkelen MT ~ 25% van de PD, terwijl cremastr...

Kleine weerstand slagaders zijn belangrijke determinanten van SVR en spelen een belangrijke rol in de pathofysiologie van vele ziekten 1,2. Aandoeningen zoals diabetes 3, zwangerschap 4, ischemie-reperfusie 5, obesitas en hypertensie 6,7 worden vaak geassocieerd met veranderde microvasculaire functie. Vasculaire myography kunnen niet alleen belangrijke inzichten in veranderingen in microvasculaire functie bij verscheidene ziekten, maar ook helpen bij het identificeren therapeutische targets en het effect van vasoactieve verbindingen te evalueren. Vaatfunctie is onderzocht met behulp van geïsoleerde kleine slagaders onder isometrisch of isobare omstandigheden vat 8. Gedetailleerde beschrijving van isometrische myography elders 9 verschaft. Er zijn echter verschillen in de gegevens die zijn verkregen uit isometrische versus isobare voorbereidingen 10-12. Aangezien onder druk arteriële voorbereidingen kan de studie van microvasculaire functie in de buurt-fysiologische omstandigheden, deverkregen bevindingen kunnen beter met in vivo gedrag van de vasculaire bed 8,13 correleren. In 1902 eerst beschreven Bayliss het effect van transmurale druk op vasculaire diameter 14. Hij merkte in kleine weerstand arteriën uit verschillende vasculaire bedden van konijnen, katten en honden die een drukafname gevolgd door vasodilatatie en een verhoging van de druk werd gevolgd door vasoconstrictie. Dit fenomeen wordt myogene reactie. Bayliss en daaropvolgende onderzoekers opgemerkt dat in isobare omstandigheden kleine weerstand slagaders ontwikkelen aanhoudende vernauwing bekend als MT 15,16. Zowel myogene reactie en MT kunnen worden bepaald door de druk myography (PM) techniek. PM wordt voornamelijk gebruikt om vaso- activiteit van kleine slagaders, aders en andere vaartuigen bepalen. Naast het effect van vasoactieve verbindingen op vasculaire diameter, PM - zoals de naam al aangeeft - wordt gebruikt om de intravasculaire druk gemedieerde ch beoordelenanges op vasculaire diameter. In de afgelopen decennia is de vooruitgang in de computer software, die verbeterde video microscopie en glazen pipet trekken PM hebben gemaakt gemakkelijker uit te voeren. Echter, dissectie van levensvatbare intacte segmenten van kleine bloedvaten blijft vervelend en soms uitdagend. Hier schetsen we een gedetailleerd protocol om myogene respons in kleine mesenteriale weerstand slagaders geïsoleerd van ratten te bestuderen.

<table border="1">
		<tbody>
		 <tr>
			<td></td>
			<td></td>
			<td></td>
			<td>Chemical</td>
		 </tr>
		 <tr>
			<td>Acetylcholine</td>
			<td>Sigma Aldrich</td>
			<td>A6625</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Calcium chloride (CaCl2)</td>
			<td>Sigma Aldrich</td>
			<td>223506</td>
			<td></td>
		 </tr>
		 <tr>
			<td>D-(+)-Glucose</td>
			<td>Sigma Aldrich</td>
			<td>G5767</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetra acetic acid (EGTA)</td>
			<td>Sigma Aldrich</td>
			<td>E3889</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Ethylene diamine tetra acetic acid (EDTA)</td>
			<td>Sigma Aldrich</td>
			<td>E9884</td>
			<td></td>
		 </tr>
		 <tr>
			<td>HEPES</td>
			<td>Sigma Aldrich</td>
			<td>H3784</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Magnesium sulfate (MgSO4)</td>
			<td>Sigma Aldrich</td>
			<td>M7506</td>
			<td></td>
		 </tr>
		 <tr>
			<td>MOPS</td>
			<td>Sigma Aldrich</td>
			<td>M5162</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Phenylephrine</td>
			<td>Sigma Aldrich</td>
			<td>P6126</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Potassium chloride (KCl)</td>
			<td>Sigma Aldrich</td>
			<td>P3911</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Potassium phosphate (KH2PO4)</td>
			<td>Sigma Aldrich</td>
			<td>P5655</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sodium bicarbonate (NaHCO3 )</td>
			<td>Sigma Aldrich</td>
			<td>S6014</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sodium chloride (NaCl)</td>
			<td>Sigma Aldrich</td>
			<td>S7653</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sodium hydroxide (NaOH)</td>
			<td>Sigma Aldrich</td>
			<td>S5881</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sodium nitroprusside</td>
			<td>Sigma Aldrich</td>
			<td>13451</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sodium phosphate monobasic monohydrate (NaH2PO4)</td>
			<td>Sigma Aldrich</td>
			<td>S9638</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sodium pyruvate</td>
			<td>Sigma Aldrich</td>
			<td>P8574</td>
			<td></td>
		 </tr>
		 <tr>
			<td></td>
			<td></td>
			<td></td>
			<td>Table 1.</td>
		 </tr>
		 <tr>
		 <td></td>
		 <td></td>
		 <td></td>
		 <td>
 <table border="1">
		<tbody>
		 <tr>
			<td>Physiological salt solution (1000 ml)</td>
			<td> mM</td>
			<td></td>
		 </tr>
		 <tr>
			<td>KCl</td>
			<td>4.9</td>
			<td>0.365 g</td>
		 </tr>
		 <tr>
			<td>NaCl</td>
			<td>112</td>
			<td>6.545 g</td>
		 </tr>
		 <tr>
			<td>MgSO4.7H2O</td>
			<td>1.2</td>
			<td>0.296 g</td>
		 </tr>
		 <tr>
			<td>KH2PO4</td>
			<td>1.2</td>
			<td>0.163 g</td>
		 </tr>
		 <tr>
			<td>Glucose</td>
			<td>11.5</td>
			<td>2.072 g</td>
		 </tr>
		 <tr>
			<td>NaHCO3</td>
			<td>26</td>
			<td>2.184 g</td>
		 </tr>
		 <tr>
			<td>HEPES</td>
			<td>10</td>
			<td>2.383 g</td>
		 </tr>
		 <tr>
			<td>CaCl2</td>
			<td>2</td>
			<td>2 ml (1M stock)</td>
		 </tr>
		 <tr>
			<td>De-ionized water</td>
			<td></td>
			<td>998 ml</td>
		 </tr>
		 <tr>
			<td>Ca2+ free physiological salt solution (100 ml)</td>
			<td> mM</td>
			<td></td>
		 </tr>
		 <tr>
			<td>KCl</td>
			<td>4.9</td>
			<td>0.036 g</td>
		 </tr>
		 <tr>
			<td>NaCl</td>
			<td>112</td>
			<td>0.645 g</td>
		 </tr>
		 <tr>
			<td>MgSO4.7H2O</td>
			<td>1.2</td>
			<td>0.029 g</td>
		 </tr>
		 <tr>
			<td>KH2PO4</td>
			<td>1.2</td>
			<td>0.016 g</td>
		 </tr>
		 <tr>
			<td>Glucose</td>
			<td>11.5</td>
			<td>0.207 g</td>
		 </tr>
		 <tr>
			<td>NaHCO3</td>
			<td>26</td>
			<td>0.218 g</td>
		 </tr>
		 <tr>
			<td>HEPES</td>
			<td>10</td>
			<td>0.238 g</td>
		 </tr>
		 <tr>
			<td>EGTA </td>
			<td>0.39</td>
			<td>0.015 g</td>
		 </tr>
		 <tr>
			<td>Sodium nitroprusside </td>
			<td>0.1</td>
			<td>0.0026 g</td>
		 </tr>
		 <tr>
			<td>De-ionized water</td>
			<td></td>
			<td>100 ml</td>
		 </tr>
		 <tr>
			<td>Dissection solution, stock (500 ml)</td>
			<td> mM</td>
			<td></td>
		 </tr>
		 <tr>
			<td>NaCl</td>
			<td>145</td>
			<td>21.18 g</td>
		 </tr>
		 <tr>
			<td>KCl</td>
			<td>4.7</td>
			<td>0.875 g</td>
		 </tr>
		 <tr>
			<td>MgSO4</td>
			<td>1.2</td>
			<td>0.739 g</td>
		 </tr>
		 <tr>
			<td>MOPS</td>
			<td>2</td>
			<td>1.049 g</td>
		 </tr>
		 <tr>
			<td>EDTA</td>
			<td>0.02</td>
			<td>0.019 g</td>
		 </tr>
		 <tr>
			<td>De-ionized water</td>
			<td></td>
			<td>500 ml</td>
		 </tr>
		 <tr>
			<td>Working dissection solution (100 ml)</td>
			<td> mM</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Dissection solution stock</td>
			<td>20 ml</td>
		 </tr>
		 <tr>
			<td>Glucose</td>
			<td>1.2</td>
			<td>0.091 g</td>
		 </tr>
		 <tr>
			<td>NaH2PO4</td>
			<td>5</td>
			<td>0.016 g</td>
		 </tr>
		 <tr>
			<td>Sodium pyruvate</td>
			<td>2</td>
			<td>0.022 g</td>
		 </tr>
		 <tr>
			<td>CaCl2</td>
			<td>2</td>
			<td>0.2 ml (1M stock)</td>
		 </tr>
		 <tr>
			<td>De-ionized water</td>
			<td></td>
			<td>79.8 ml</td>
		 </tr>
	 </tbody>
</table>
 </td>
	 </tr>
		 <tr>
		 <td></td>
		 <td></td>
		 <td></td>
		 <td>Table 2. Composition of Experimetnal solutions</td>
	 </tr>
		 <tr>
			<td></td>
			<td></td>
			<td></td>
			<td>Equipment</td>
		 </tr>
		 <tr>
			<td>CCD Monochrome Camera</td>
			<td>The imaging Source</td>
			<td>DMK 21AU04</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Single inline solution heater</td>
			<td>Warner Instruments</td>
			<td>64-0102</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Thermistor</td>
			<td>Warner Instruments</td>
			<td>64-0108</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Dual automatic temperature controller</td>
			<td>Warner Instruments</td>
			<td>TC-344B</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Flaming/Brown micropipette puller</td>
			<td>Sutter Instruments </td>
			<td>P-97</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Fluorescence System Interface</td>
			<td>IonOptix</td>
			<td>model FSI-700</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Forceps and scissors </td>
			<td>World Precision Instruments</td>
			<td></td>
			<td></td>
		 </tr>
		 <tr>
			<td>Ion Wizard-Core and Analysis</td>
			<td>IonOptix</td>
			<td>Ion Wizard 6.0</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Laboratory tubing</td>
			<td>Silastic </td>
			<td>508-005</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Male Sprague Dawley rat</td>
			<td>Harlan Laboratories</td>
			<td></td>
			<td></td>
		 </tr>
		 <tr>
			<td>Master flex console drive</td>
			<td>Cole-parmer</td>
			<td></td>
			<td></td>
		 </tr>
		 <tr>
			<td>Milli-Q Plus Ultrapure Water System</td>
			<td>Millipore</td>
			<td>ZD5211584</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Ophthalmic monofilament nylon suture </td>
			<td>Ethicon </td>
			<td>9007G</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Photometry and Dimensioning Microscope</td>
			<td>Motic</td>
			<td>AE31</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Pressure Servo Controller with peristaltic pump and pressure transducer</td>
			<td>Living Systems Instrumentation</td>
			<td>PS-200</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Stereomicroscope</td>
			<td>Nikon Instruments Inc</td>
			<td>SMZ660</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Vessel Chamber</td>
			<td>Living Systems Instrumentation</td>
			<td>CH-1</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Dissection dish</td>
			<td>Living Systems Instrumentation</td>
			<td>DD-90-S</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Thin Wall Glass Capillaries</td>
			<td>World Precision Instruments</td>
			<td>TW120-6</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Microforge</td>
			<td>Stoelting</td>
			<td>51550</td>
			<td></td>
		 </tr>
		 <tr>
			<td></td>
			<td></td>
			<td></td>
			<td>Table 3.</td>
		 </tr>
	 </tbody>
</table>

assessing myogenic response and vasoactivity in resistance mesenteric arteries using pressure myography

Kleine weerstand slagaders vernauwen en verwijden respectievelijk bij toenemende intraluminale of verlaagde druk; dit fenomeen bekend als myogene respons is een belangrijke regulator van de lokale doorbloeding. In isobare omstandigheden kleine weerstand slagaders ontwikkelen aanhoudende vernauwing bekend als myogene toon (MT), dat is een belangrijke determinant van de systemische vasculaire weerstand (SVR). Vandaar, ex vivo onder druk bereidingen van kleine weerstand slagaders zijn belangrijke instrumenten om microvasculaire functie te bestuderen in de buurt-fysiologische toestanden. Hiertoe wordt een vers geïsoleerde intacte segment van een kleine weerstand arterie (diameter ~ 260 urn) aangebracht op twee kleine glazen canules en onder druk. Deze arteriële voorbereidingen behouden meest in vivo kenmerken en vergunning beoordeling van vasculaire tonus in real-time. Hier bieden we een gedetailleerd protocol voor het beoordelen van vasoactiviteit in onder druk kleine weerstand mesenterica slagaders van ratten; deze slagaders ontwikkelinglangdurige vasoconstrictie - ongeveer 25% van de maximale diameter - bij een druk van 70 mmHg. Deze arteriële preparaten kunnen worden gebruikt om het effect van de onderzochte verbindingen op tussen intra-arteriële druk en vaatactiviteit onderzoeken en vaststellen veranderingen in microvasculaire functie bij diermodellen van verschillende ziekten.

Schematische weergave van een typische druk myograaf opstelling is weergegeven in figuur 1. De twee uiteinden van het vaartuig canule met een glazen micropipet en vastgezet met hechtingen aan beide zijden. Via slangen en een open kraan, wordt een canule verbonden met een servo-gestuurde druk-regulator; de andere canule is verbonden met een gesloten kraan. De kamer wordt geperfuseerd met PSS en vasculaire diameter zijn beschadigd door een omgekeerde microscoop verbonden met een CCD camera. <p class="...

Watch this Scientific Journal Video about Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography at JoVE.com

Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography

Pressure myography wordt gebruikt om vasoactiviteit van kleine slagaders die aanhoudende vernauwing ontwikkelen wanneer onder druk te beoordelen. Het manuscript geeft een gedetailleerd protocol te beoordelen geïsoleerde segmenten van kleine mesenterische arteriën van ratten vaatactiviteit en het effect van de intraluminale druk op de vasculaire diameter.

Het beoordelen Myogene Response en vasoactiviteit In Resistance Mesenteriale slagaders behulp Pressure myography

Small resistance arteries constrict and dilate respectively in response to increased or decreased intraluminal pressure; this phenomenon known as myogenic ...

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