Name: assessing myogenic response and vasoactivity in resistance mesenteric arteries using pressure myography
Uploaded: 2015-07-06T13:00:00
Description: Watch this Scientific Journal Video about Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography at JoVE.com

Sandeep Khurana è supportato dal NIH (K08DKO81479). Vikrant Rachakonda è supportato da (T32DK067872).

Gli esempi qui riportati sono da esperimenti approvati dal IACUC presso la Georgia Regents University - Protocollo n: # 2011-0408 1. Preparazione dei reagenti <ol><li> Preparare la soluzione dissezione magazzino: per 500 ml di soluzione madre dissezione (5x), sciogliere 21,18 g NaCl, 0,875 g KCl, 0,739 g MgSO4, 1.049 g MOPS e 0.019 g di EDTA in 450 ml di acqua Milli-Q. Regolare il pH a 7,3-7,4 con 1 N NaOH. Portare al volume di 500 ml con acqua Milli-Q. Soluzione madre può esse...

<ol>
	<li>Christensen, K. L., Mulvany, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Location+of+resistance+arteries.">Location of resistance arteries.</a> J Vasc Res. 38, 1-12 (2001).</li><li>Rietzschel, E. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Oxidized+low-density+lipoprotein+cholesterol+is+associated+with+decreases+in+cardiac+function+independent+of+vascular+alterations.">Oxidized low-density lipoprotein cholesterol is associated with decreases in cardiac function independent of vascular alterations.</a> Hypertension. 52, 535-541 (2008).</li><li>Hill, M. A., Ege, E. 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L., Lessov, N., Porter, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reperfusion+decreases+myogenic+reactivity+and+alters+middle+cerebral+artery+function+after+focal+cerebral+ischemia+in+rats.">Reperfusion decreases myogenic reactivity and alters middle cerebral artery function after focal cerebral ischemia in rats.</a> Stroke. 28, 176-180 (1997).</li><li>Ren, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enhanced+myogenic+response+in+the+afferent+arteriole+of+spontaneously+hypertensive+rats.">Enhanced myogenic response in the afferent arteriole of spontaneously hypertensive rats.</a> Am J Physiol Heart Circ Physiol. 298, H1769-H1775 (2010).</li><li>Hayashi, K., Epstein, M., Saruta, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Altered+myogenic+responsiveness+of+the+renal+microvasculature+in+experimental+hypertension.">Altered myogenic responsiveness of the renal microvasculature in experimental hypertension.</a> J Hypertens. 14, 1387-1401 (1996).</li><li>Schubert, R., Mulvany, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+myogenic+response:+established+facts+and+attractive+hypotheses.">The myogenic response: established facts and attractive hypotheses.</a> Clin Sci (Lond). 96, 313-326 (1999).</li><li>Bridges, L. E., Williams, C. L., Pointer, M. A., Awumey, E. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mesenteric+artery+contraction+and+relaxation+studies+using+automated+wire+myography.">Mesenteric artery contraction and relaxation studies using automated wire myography.</a> J Vis Exp. , (2011).</li><li>Buus, N. H., Vanbavel, E., Mulvany, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differences+in+Sensitivity+of+Rat+Mesenteric+Small+Arteries+to+Agonists+When+Studied+as+Ring+Preparations+or+as+Cannulated+Preparations.">Differences in Sensitivity of Rat Mesenteric Small Arteries to Agonists When Studied as Ring Preparations or as Cannulated Preparations.</a> British Journal of Pharmacology. 112, 579-587 (1994).</li><li>Falloon, B. J., Stephens, N., Tulip, J. R., Heagerty, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+small+artery+sensitivity+and+morphology+in+pressurized+and+wire-mounted+preparations.">Comparison of small artery sensitivity and morphology in pressurized and wire-mounted preparations.</a> Am J Physiol. 268, H670-H678 (1995).</li><li>Schubert, R., Wesselman, J. P. M., Nilsson, H., Mulvany, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noradrenaline-induced+depolarization+is+smaller+in+isobaric+compared+to+isometric+preparations+of+rat+mesenteric+small+arteries.">Noradrenaline-induced depolarization is smaller in isobaric compared to isometric preparations of rat mesenteric small arteries.</a> Pflugers Archiv-European Journal of Physiology. 431, 794-796 (1996).</li><li>Khurana, S., Raina, H., Pappas, V., Raufman, J. P., Pallone, T. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+deoxycholylglycine,+a+conjugated+secondary+bile+acid,+on+myogenic+tone+and+agonist-induced+contraction+in+rat+resistance+arteries.">Effects of deoxycholylglycine, a conjugated secondary bile acid, on myogenic tone and agonist-induced contraction in rat resistance arteries.</a> PLoS One. 7, e32006 (2012).</li><li>Bayliss, W. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=On+the+local+reactions+of+the+arterial+wall+to+changes+of+internal+pressure.">On the local reactions of the arterial wall to changes of internal pressure.</a> J Physiol. 28, 220-231 (1902).</li><li>Hughes, J. M., Bund, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Arterial+myogenic+properties+of+the+spontaneously+hypertensive+rat.">Arterial myogenic properties of the spontaneously hypertensive rat.</a> Exp Physiol. 87, 527-534 (2002).</li><li>Mulvany, M. J., Halpern, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanical+properties+of+vascular+smooth+muscle+cells+in+situ.">Mechanical properties of vascular smooth muscle cells in situ.</a> Nature. 260, 617-619 (1976).</li><li>Raina, H., Zacharia, J., Li, M., Wier, W. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Activation+by+Ca2+/calmodulin+of+an+exogenous+myosin+light+chain+kinase+in+mouse+arteries.">Activation by Ca2+/calmodulin of an exogenous myosin light chain kinase in mouse arteries.</a> J Physiol. 587, 2599-2612 (2009).</li><li>Fenger-Gron, J., Mulvany, M. J., Christensen, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mesenteric+blood+pressure+profile+of+conscious,+freely+moving+rats.">Mesenteric blood pressure profile of conscious, freely moving rats.</a> J Physiol. 488 (Pt 3), 753-760 (1995).</li><li>Davis, M. J., Hill, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Signaling+mechanisms+underlying+the+vascular+myogenic+response).">Signaling mechanisms underlying the vascular myogenic response).</a> Physiol Rev. 79, 387-423 (1999).</li><li>Osmond, J. M., Mintz, J. D., Dalton, B., Stepp, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Obesity+increases+blood+pressure,+cerebral+vascular+remodeling,+and+severity+of+stroke+in+the+Zucker+rat.">Obesity increases blood pressure, cerebral vascular remodeling, and severity of stroke in the Zucker rat.</a> Hypertension. 53, 381-386 (2009).</li><li>Ge, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endogenously+produced+20-HETE+modulates+myogenic+and+TGF+response+in+microperfused+afferent+arterioles.">Endogenously produced 20-HETE modulates myogenic and TGF response in microperfused afferent arterioles.</a> Prostaglandins Other Lipid Mediat. 102-103, 42-48 (2013).</li><li>Ryan, M. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Placental+ischemia+impairs+middle+cerebral+artery+myogenic+responses+in+the+pregnant+rat.">Placental ischemia impairs middle cerebral artery myogenic responses in the pregnant rat.</a> Hypertension. 58, 1126-1131 (2011).</li><li>Bagi, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Type+2+diabetic+mice+have+increased+arteriolar+tone+and+blood+pressure:+enhanced+release+of+COX-2-derived+constrictor+prostaglandins.">Type 2 diabetic mice have increased arteriolar tone and blood pressure: enhanced release of COX-2-derived constrictor prostaglandins.</a> Arterioscler Thromb Vasc Biol. 25, 1610-1616 (2005).</li></ol>

Passaggi critici, risoluzione dei problemi e modifiche In una preparazione tipica imbarcazione isobarica, l&#39;arteria viene pressurizzato a 70 mmHg tra due cannule di vetro perfusi con acqua calda (37 ° C) PSS. Dopo 30-45 min, arterie sviluppano MT, caratterizzato dalla riduzione spontanea di diametro che stabilizza in 20-30 min. Le arterie di resistenza dai vari distretti vascolari sviluppano MT variabile. Per esempio resistenza ratto arterie mesenteriche sviluppano MT ~ 25% di PD, mentre l...

Arterie piccola resistenza sono i principali determinanti di SVR e svolgono un ruolo importante nella fisiopatologia di molte malattie 1,2. Condizioni come il diabete 3, la gravidanza 4, ischemia-riperfusione 5, obesità e ipertensione 6,7 sono spesso associati con la funzione microvascolare alterata. Myography vascolare può fornire non solo importanti conoscenze cambiamenti nella funzione microvascolare in varie malattie, ma anche aiutare a identificare target terapeutici e di valutare l&#39;efficacia di composti vasoattivi. Funzione vascolare è stata studiata utilizzando isolate piccole arterie in condizioni di nave isometriche o isobariche 8. Descrizione dettagliata di myography isometrica è fornito altrove 9. Tuttavia ci sono differenze tra i dati ottenuti da isometrica contro preparazioni isobariche 10-12. Poiché preparazioni arteriosi pressurizzati permettono lo studio della funzione microvascolare in condizioni pressoché fisiologiche, larisultati ottenuti possono correlare meglio con il comportamento in vivo del letto vascolare 8,13. Nel 1902 Bayliss descrisse per primo l&#39;effetto della pressione transmurale del diametro vascolare 14. Si osserva in piccole arterie di resistenza di vari distretti vascolari di conigli, cani e gatti che una diminuzione della pressione è stata seguita da vasodilatazione, e un aumento della pressione è stata seguita da vasocostrizione. Questo fenomeno è noto come risposta miogenico. Bayliss e gli investigatori successivi hanno osservato che in condizioni isobariche arterie piccola resistenza sviluppano costrizione sostenuta chiamato MT 15,16. Sia risposta miogenica e MT possono essere valutati utilizzando myography pressione (PM) tecnica. PM viene utilizzato principalmente per determinare vasoactivity delle piccole arterie, vene e altre imbarcazioni. Oltre a valutare l&#39;effetto dei composti vasoattivi sul diametro vascolare, PM - come indica il nome - viene utilizzato per valutare intravascolare ch-mediata pressioneanges del diametro vascolare. Nel corso degli ultimi decenni i progressi nel software per computer, che ha migliorato la microscopia video e pipetta di vetro tirando, hanno reso PM più facile da eseguire. Tuttavia, la dissezione di segmenti intatti vitali di piccoli vasi sanguigni rimane noiosa e talvolta impegnativo. Qui descriviamo un protocollo dettagliato per studiare la risposta miogenico nelle piccole arterie di resistenza mesenteriche isolati da ratti.

<table border="1">
		<tbody>
		 <tr>
			<td></td>
			<td></td>
			<td></td>
			<td>Chemical</td>
		 </tr>
		 <tr>
			<td>Acetylcholine</td>
			<td>Sigma Aldrich</td>
			<td>A6625</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Calcium chloride (CaCl2)</td>
			<td>Sigma Aldrich</td>
			<td>223506</td>
			<td></td>
		 </tr>
		 <tr>
			<td>D-(+)-Glucose</td>
			<td>Sigma Aldrich</td>
			<td>G5767</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetra acetic acid (EGTA)</td>
			<td>Sigma Aldrich</td>
			<td>E3889</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Ethylene diamine tetra acetic acid (EDTA)</td>
			<td>Sigma Aldrich</td>
			<td>E9884</td>
			<td></td>
		 </tr>
		 <tr>
			<td>HEPES</td>
			<td>Sigma Aldrich</td>
			<td>H3784</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Magnesium sulfate (MgSO4)</td>
			<td>Sigma Aldrich</td>
			<td>M7506</td>
			<td></td>
		 </tr>
		 <tr>
			<td>MOPS</td>
			<td>Sigma Aldrich</td>
			<td>M5162</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Phenylephrine</td>
			<td>Sigma Aldrich</td>
			<td>P6126</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Potassium chloride (KCl)</td>
			<td>Sigma Aldrich</td>
			<td>P3911</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Potassium phosphate (KH2PO4)</td>
			<td>Sigma Aldrich</td>
			<td>P5655</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sodium bicarbonate (NaHCO3 )</td>
			<td>Sigma Aldrich</td>
			<td>S6014</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sodium chloride (NaCl)</td>
			<td>Sigma Aldrich</td>
			<td>S7653</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sodium hydroxide (NaOH)</td>
			<td>Sigma Aldrich</td>
			<td>S5881</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sodium nitroprusside</td>
			<td>Sigma Aldrich</td>
			<td>13451</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sodium phosphate monobasic monohydrate (NaH2PO4)</td>
			<td>Sigma Aldrich</td>
			<td>S9638</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sodium pyruvate</td>
			<td>Sigma Aldrich</td>
			<td>P8574</td>
			<td></td>
		 </tr>
		 <tr>
			<td></td>
			<td></td>
			<td></td>
			<td>Table 1.</td>
		 </tr>
		 <tr>
		 <td></td>
		 <td></td>
		 <td></td>
		 <td>
 <table border="1">
		<tbody>
		 <tr>
			<td>Physiological salt solution (1000 ml)</td>
			<td> mM</td>
			<td></td>
		 </tr>
		 <tr>
			<td>KCl</td>
			<td>4.9</td>
			<td>0.365 g</td>
		 </tr>
		 <tr>
			<td>NaCl</td>
			<td>112</td>
			<td>6.545 g</td>
		 </tr>
		 <tr>
			<td>MgSO4.7H2O</td>
			<td>1.2</td>
			<td>0.296 g</td>
		 </tr>
		 <tr>
			<td>KH2PO4</td>
			<td>1.2</td>
			<td>0.163 g</td>
		 </tr>
		 <tr>
			<td>Glucose</td>
			<td>11.5</td>
			<td>2.072 g</td>
		 </tr>
		 <tr>
			<td>NaHCO3</td>
			<td>26</td>
			<td>2.184 g</td>
		 </tr>
		 <tr>
			<td>HEPES</td>
			<td>10</td>
			<td>2.383 g</td>
		 </tr>
		 <tr>
			<td>CaCl2</td>
			<td>2</td>
			<td>2 ml (1M stock)</td>
		 </tr>
		 <tr>
			<td>De-ionized water</td>
			<td></td>
			<td>998 ml</td>
		 </tr>
		 <tr>
			<td>Ca2+ free physiological salt solution (100 ml)</td>
			<td> mM</td>
			<td></td>
		 </tr>
		 <tr>
			<td>KCl</td>
			<td>4.9</td>
			<td>0.036 g</td>
		 </tr>
		 <tr>
			<td>NaCl</td>
			<td>112</td>
			<td>0.645 g</td>
		 </tr>
		 <tr>
			<td>MgSO4.7H2O</td>
			<td>1.2</td>
			<td>0.029 g</td>
		 </tr>
		 <tr>
			<td>KH2PO4</td>
			<td>1.2</td>
			<td>0.016 g</td>
		 </tr>
		 <tr>
			<td>Glucose</td>
			<td>11.5</td>
			<td>0.207 g</td>
		 </tr>
		 <tr>
			<td>NaHCO3</td>
			<td>26</td>
			<td>0.218 g</td>
		 </tr>
		 <tr>
			<td>HEPES</td>
			<td>10</td>
			<td>0.238 g</td>
		 </tr>
		 <tr>
			<td>EGTA </td>
			<td>0.39</td>
			<td>0.015 g</td>
		 </tr>
		 <tr>
			<td>Sodium nitroprusside </td>
			<td>0.1</td>
			<td>0.0026 g</td>
		 </tr>
		 <tr>
			<td>De-ionized water</td>
			<td></td>
			<td>100 ml</td>
		 </tr>
		 <tr>
			<td>Dissection solution, stock (500 ml)</td>
			<td> mM</td>
			<td></td>
		 </tr>
		 <tr>
			<td>NaCl</td>
			<td>145</td>
			<td>21.18 g</td>
		 </tr>
		 <tr>
			<td>KCl</td>
			<td>4.7</td>
			<td>0.875 g</td>
		 </tr>
		 <tr>
			<td>MgSO4</td>
			<td>1.2</td>
			<td>0.739 g</td>
		 </tr>
		 <tr>
			<td>MOPS</td>
			<td>2</td>
			<td>1.049 g</td>
		 </tr>
		 <tr>
			<td>EDTA</td>
			<td>0.02</td>
			<td>0.019 g</td>
		 </tr>
		 <tr>
			<td>De-ionized water</td>
			<td></td>
			<td>500 ml</td>
		 </tr>
		 <tr>
			<td>Working dissection solution (100 ml)</td>
			<td> mM</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Dissection solution stock</td>
			<td>20 ml</td>
		 </tr>
		 <tr>
			<td>Glucose</td>
			<td>1.2</td>
			<td>0.091 g</td>
		 </tr>
		 <tr>
			<td>NaH2PO4</td>
			<td>5</td>
			<td>0.016 g</td>
		 </tr>
		 <tr>
			<td>Sodium pyruvate</td>
			<td>2</td>
			<td>0.022 g</td>
		 </tr>
		 <tr>
			<td>CaCl2</td>
			<td>2</td>
			<td>0.2 ml (1M stock)</td>
		 </tr>
		 <tr>
			<td>De-ionized water</td>
			<td></td>
			<td>79.8 ml</td>
		 </tr>
	 </tbody>
</table>
 </td>
	 </tr>
		 <tr>
		 <td></td>
		 <td></td>
		 <td></td>
		 <td>Table 2. Composition of Experimetnal solutions</td>
	 </tr>
		 <tr>
			<td></td>
			<td></td>
			<td></td>
			<td>Equipment</td>
		 </tr>
		 <tr>
			<td>CCD Monochrome Camera</td>
			<td>The imaging Source</td>
			<td>DMK 21AU04</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Single inline solution heater</td>
			<td>Warner Instruments</td>
			<td>64-0102</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Thermistor</td>
			<td>Warner Instruments</td>
			<td>64-0108</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Dual automatic temperature controller</td>
			<td>Warner Instruments</td>
			<td>TC-344B</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Flaming/Brown micropipette puller</td>
			<td>Sutter Instruments </td>
			<td>P-97</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Fluorescence System Interface</td>
			<td>IonOptix</td>
			<td>model FSI-700</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Forceps and scissors </td>
			<td>World Precision Instruments</td>
			<td></td>
			<td></td>
		 </tr>
		 <tr>
			<td>Ion Wizard-Core and Analysis</td>
			<td>IonOptix</td>
			<td>Ion Wizard 6.0</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Laboratory tubing</td>
			<td>Silastic </td>
			<td>508-005</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Male Sprague Dawley rat</td>
			<td>Harlan Laboratories</td>
			<td></td>
			<td></td>
		 </tr>
		 <tr>
			<td>Master flex console drive</td>
			<td>Cole-parmer</td>
			<td></td>
			<td></td>
		 </tr>
		 <tr>
			<td>Milli-Q Plus Ultrapure Water System</td>
			<td>Millipore</td>
			<td>ZD5211584</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Ophthalmic monofilament nylon suture </td>
			<td>Ethicon </td>
			<td>9007G</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Photometry and Dimensioning Microscope</td>
			<td>Motic</td>
			<td>AE31</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Pressure Servo Controller with peristaltic pump and pressure transducer</td>
			<td>Living Systems Instrumentation</td>
			<td>PS-200</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Stereomicroscope</td>
			<td>Nikon Instruments Inc</td>
			<td>SMZ660</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Vessel Chamber</td>
			<td>Living Systems Instrumentation</td>
			<td>CH-1</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Dissection dish</td>
			<td>Living Systems Instrumentation</td>
			<td>DD-90-S</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Thin Wall Glass Capillaries</td>
			<td>World Precision Instruments</td>
			<td>TW120-6</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Microforge</td>
			<td>Stoelting</td>
			<td>51550</td>
			<td></td>
		 </tr>
		 <tr>
			<td></td>
			<td></td>
			<td></td>
			<td>Table 3.</td>
		 </tr>
	 </tbody>
</table>

assessing myogenic response and vasoactivity in resistance mesenteric arteries using pressure myography

Le piccole arterie di resistenza restringono e si dilatano rispettivamente in risposta ad un aumento o diminuzione della pressione endoluminale; questo fenomeno noto come risposta miogenico è un regolatore chiave di flusso ematico locale. In condizioni isobariche piccole arterie di resistenza sviluppano sostenuti costrizione noto come tono miogenico (MT), che è un importante determinante di resistenze vascolari sistemiche (SVR). Quindi, ex vivo preparazioni pressione di piccole arterie di resistenza sono i principali strumenti per studiare la funzione microvascolare negli stati vicini-fisiologico. Per ottenere questo, un segmento intatto fresco isolato di una piccola arteria resistenza (diametro ~ 260 micron) è montata su due cannule piccolo vetro e pressurizzato. Queste preparazioni arteriose conservano più a caratteristiche vivo e la valutazione permesso del tono vascolare in tempo reale. Qui forniamo un protocollo dettagliato per la valutazione vasoactivity in pressurizzati piccole arterie resistenza mesenterici di ratti; queste arterie sviluppanovasocostrizione sostenuta - circa il 25% del diametro massimo - quando sotto pressione a 70 mmHg. Queste preparazioni arteriose possono essere utilizzati per studiare l&#39;effetto dei composti in fase di sperimentazione in relazione tra pressione intra-arteriosa e vasoactivity e determinare alterazioni della funzione microvascolare in modelli animali di varie malattie.

Rappresentazione schematica di un miografo pressione tipico set-up è mostrata in Figura 1. Le due estremità del recipiente sono cannulati con una micropipetta di vetro e fissati con suture su entrambi i lati. Via tubo e un rubinetto aperto, una cannula viene collegato ad un regolatore di pressione servocomandato; l&#39;altra cannula è collegata ad un rubinetto chiuso. La camera è perfuso con PSS e diametro vascolare cambiamenti sono osservati da un microscopio invertito collegato ad una telecamera C...

Watch this Scientific Journal Video about Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography at JoVE.com

Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography

Myography pressione è utilizzato per valutare vasoactivity di piccole arterie che si sviluppano costrizione sostenuta quando sotto pressione. Questo manoscritto fornisce un protocollo dettagliato per valutare in segmenti isolati di piccole arterie mesenterici di ratto, vasoactivity e l&#39;effetto della pressione intraluminale del diametro vascolare.

Valutare Miogenica risposta e Vasoactivity nelle arterie di resistenza mesenteriche Utilizzando Myography Pressione

Small resistance arteries constrict and dilate respectively in response to increased or decreased intraluminal pressure; this phenomenon known as myogenic ...

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