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DOI :
10.3791/50998-v
•
11:46 min
February 7th, 2014
Chapters
0:05
Title
1:47
Preparing the PDMS Chip
3:47
Using the Larva Chip for Live Imaging
7:23
Inducing a Nerve Crush Injury to Larval Segmental Nerves
8:42
Immobilization Enhanced Imaging
10:25
Conclusion
果蝇幼虫对于实时成像,由于其半透明的角质层和强大的遗传学一个有吸引力的模型系统。本协议描述了如何利用单层PDMS设备,被称为“幼虫芯片的细胞过程的3 龄幼虫果蝇的神经元内的实时成像。
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