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DOI :
10.3791/51005-v
January 30th, 2014
Chapters
0:05
Title
1:19
Preparation
2:23
Sample Collection
4:31
Growing Cortical Cells
5:56
Isolation of Microglia from Mixed Glial Culture
7:30
Results: Preserved Immunophenotype and Functionality in Isolated Microglia
8:46
Conclusion
一键小胶质细胞生物学的研究成功是小胶质细胞免疫功能的体外从中枢神经系统组织分离过程中的保存。通过旋转振动造成的高纯度和immunofunctional细胞培养物中分离的小胶质细胞所评估的荧光成像,免疫细胞化学,ELISA和以下的小胶质细胞活化的促炎性刺激脂多糖(LPS)和Pam 3 CSK 4(PAM)。
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