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DOI :
10.3791/51030-v
February 24th, 2014
Chapters
0:05
Title
1:26
Whitefly, Plant, and Virus Preparations
2:10
Whole Mount FISH of Whiteflies
3:58
Whitefly Dissection Followed by TYLCV FISH
6:40
Plant Handling, Hand-sectioning, Fixation, and Hybridization for TYLCV FISH
8:29
Results: FISH Images
10:16
Conclusion
ここではin situハイブリダイゼーションの単純な蛍光(FISH)昆虫や植物組織中のウイルスや細菌の局在のための方法を説明します。このプロトコルは、全体のマウントおよび顕微鏡用切片におけるmRNAの可視化のために拡張することができます。
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