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DOI :
10.3791/51030-v
February 24th, 2014
Chapters
0:05
Title
1:26
Whitefly, Plant, and Virus Preparations
2:10
Whole Mount FISH of Whiteflies
3:58
Whitefly Dissection Followed by TYLCV FISH
6:40
Plant Handling, Hand-sectioning, Fixation, and Hybridization for TYLCV FISH
8:29
Results: FISH Images
10:16
Conclusion
우리는 곤충과 식물 조직에서 바이러스와 박테리아의 현지화 여기 현장 하이브리드 화에 간단한 형광 (FISH) 방법을 설명합니다. 이 프로토콜은 전체 산에서의 mRNA의 시각화 및 미세한 부분에 대한 확장 할 수 있습니다.
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