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DOI :
10.3791/51040-v
March 11th, 2014
Chapters
0:05
Title
1:32
Agar Bridge and Cut-open Rig Preparation
3:54
Cut-open Procedure
9:48
Results: The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique with Fluorometry
10:27
Conclusion
剖切开凡士林差距的方法来获得电压依赖性离子通道的离子和门控电流的表达在非洲爪蟾卵母细胞具有高分辨率的快速通道动力学低噪音录音。有轻微的修改,电压钳荧光可以被耦合到所述剖切开的卵母细胞的协议。
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