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DOI :
10.3791/51052-v
•
7:03 min
January 31st, 2014
Chapters
0:05
Title
1:25
Photo-activation and IV Injection of Caged Collagen Mimetic Peptides (CMPs)
2:22
Near Infrared (NIR) Fluorescence Imaging of Skeletal Collagen Remodeling Activity In vivo
3:31
Visualization of Collagens in Ex vivo Tissue Sections
5:19
Representative Images
6:28
Conclusion
この手順では、IR、蛍光マウスでのコラーゲンのリモデリング活動の画像化だけでなく、変性コラーゲンの鎖とハイブリダイズするために、光トリガすることができるケージコラーゲン模倣ペプチドを使用して、組織切片におけるコラーゲンのex vivo染色に近い生体内で示しています。
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