Name: detecting abnormalities in choroidal vasculature in a mouse model of age-related macular degeneration by time-course indocyanine green angiography
Uploaded: 2014-02-19T15:00:00
Description: Watch this Scientific Journal Video about Detecting Abnormalities in Choroidal Vasculature in a Mouse Model of Age-related Macular Degeneration by Time-course Indocyanine Green Angiography at JoVE.com

Dette arbejde blev støttet af NIH tilskud 1R01EY022901, Career Development Award fra forskning til forebygge blindhed (RPB), CMReeves &amp; MA Reeves Foundation, E. Matilda Ziegler Foundation for the Blind, Tempelridderne Eye Foundation, og en ubegrænset bevilling til Institut for oftalmologi ved University of Utah fra RPB. Vi takker Balamurali Ambati til teknisk bistand på Spectralis multimodalitet Imaging System og Tao Zhang for diskussioner og kommentarer til manuskriptet.

1.. Udarbejdelse af instrumenter <ol><li> Proceduren udføres i en procedure rum i et dyr facilitet. </li><li> Bær dykkermasker, hår huer, kirurgiske kjoler, sterile mund-covers og handsker, før du begynder eksperimentet. </li><li> Varm vand i et bægerglas til ~ 40 ° C på en kogeplade. </li><li> Placer en steril blå pude oven på en varmepude, der senere vil blive anvendt til at fastholde musen kropstemperatur under billedbehandling. Tænd varmepude. </li><li> Forbered Imaging System: <ol><li> Fjern støvhætte...

<ol>
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F., Heiduschka, P., Eter, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Retinal+fundus+imaging+in+mouse+models+of+retinal+diseases.">Retinal fundus imaging in mouse models of retinal diseases.</a> Methods Mol. Biol. 935, 41-67 (2013).</li><li>Seeliger, M. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+confocal+imaging+of+the+retina+in+animal+models+using+scanning+laser+ophthalmoscopy.">In vivo confocal imaging of the retina in animal models using scanning laser ophthalmoscopy.</a> Vision Res. 45, 3512-3519 (2005).</li><li>Fischer, M. D., Zhour, A., Kernstock, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phenotyping+of+mouse+models+with+OCT.">Phenotyping of mouse models with OCT.</a> Methods Mol. Biol. 935, 79-85 (2013).</li><li>Jian, Y., Zawadzki, R. J., Sarunic, M. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adaptive+optics+optical+coherence+tomography+for+in+vivo+mouse+retinal+imaging.">Adaptive optics optical coherence tomography for in vivo mouse retinal imaging.</a> J. Biomed. Opt. 18, 56007 (2013).</li><li>Ciardella, A. P., Donsoff, I. M., Huang, S. J., Costa, D. L., Yannuzzi, L. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polypoidal+choroidal+vasculopathy.">Polypoidal choroidal vasculopathy.</a> Surv. 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A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polypoidal+choroidal+vasculopathy+masquerading+as+central+serous+chorioretinopathy.">Polypoidal choroidal vasculopathy masquerading as central serous chorioretinopathy.</a> Ophthalmology. 107, 767-777 (2000).</li><li>Janssen, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Abnormal+vessel+formation+in+the+choroid+of+mice+lacking+tissue+inhibitor+of+metalloprotease-3.">Abnormal vessel formation in the choroid of mice lacking tissue inhibitor of metalloprotease-3.</a> Invest. Ophthalmol. Vis. Sci. 49, 2812-2822 (2008).</li><li>Ding, X., Patel, M., Chan, C. 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I denne undersøgelse har vi vist, at brug af ICGA til billede choroidale læsioner i HTRA1 transgene mus. Kendetegnene for de tidlige, midterste og sene faser af ICGA i vores musemodel matche tidsforløbet godt i humane undersøgelser 1.. Det er vigtigt at gøre bedre sammenligninger mellem menneskelige patologi og animalske fænotyper, som er uvurderlig for forskning på patofysiologiske mekanismer og behandling strategier for forhold relateret til årehinden som AMD. Vi først ...

Indocyaningrønt angiografi (ICGA) er en diagnostisk test til billede problemer relateret til blodkarrene i øjet. Absorptionsspektret for ICG spænder fra 790 til 805 nm, mens emissionsspektret spænder fra 770 til 880 nm med topemission ved 835 nm 1. Dette er forskelligt fra de andre populære farvestof, natriumfluorescein, hvis frekvenser falder i det synlige område. Det infrarøde spektrum giver ICG at gennemtrænge retinale pigmentepitel (RPE), serosanguineous væske og lipid ekssudater, som alle nemt kan blokere visualisering af natrium-fluorescein baseret fluoresceinangiografi (FA). ICG er 98% proteinbundet i karrene resulterer i mindre ekstravasation tillader forbedret billeddannelse af choroidale fartøjer og choroidale læsioner 1,2. ICGA er næsten det eneste valg at visualisere choroidal vaskulatur, som er posteriort RPE. Figur 1 viser sammenligning af ICGA og FA i billedbehandling vaskulaturen i muse øjne. FA kan be brugt til billede retinal vaskulatur godt, men ikke den choroidal kar. I modsætning hertil kan ICGA anvendes til billedet både nethinden og årehinden vaskulatur. ICGA udføres med høj opløsning digital billedbehandling systemer eller scanning laseroftalmoskoper (SLO) sammen med infrarød-følsomme videokameraer, som vi vil bruge i denne undersøgelse. I klinikken har ICGA blevet anbefalet at diagnosticere en række chorioretinal lidelser i forbindelse med koroidale vaskulaturen herunder Polypoidal Choroidal vaskulopati (PCV), Retinal angiomatous spredning (RAP), angioid striber, vitelliform makulært dystrofi, central serøs chorioretinopati, koroidal hemangioma, hemorrhaging retinal arteriolære macroaneurysms, koroidale tumorer og visse former for posterior uveitis 1,3. Kombinationen af ​​ICGA med FA og optisk kohærenstomografi (OLT) giver stærke redskaber til klinikerne i diagnosticering og behandling af eksudativ aldersrelateret maculadegeneration (AMD) 4-10. ICGA er især nyttigt til at diagnosticere tilstande, der involverer årehinden. Faktisk er ICGA betragtes som den gyldne standard til diagnosticering af PCV, en variant af exudativ AMD 11-13. PCV er kendetegnet ved et netværk af forgrening fartøjer med terminal polypoidal dilations i choroidale vasculature 11-13. PCV er ofte forbundet med tilbagevendende serosanguineous afdelinger af RPE og nethinden med lækage og blødning fra polypoidal komponenter 11,14,15. Vi har for nylig rapporteret dannelsen af den første PCV dyremodel af transgent udtrykker human HTRA1, en ​​multifunktionel serinprotease, i muse retinale pigmentepitel (RPE) 16. Vi viste, at øget HTRA1 inducerede karakteristiske træk ved PCV, f.eks polypoidal læsioner. Her viste vi brug af tidsforløbet ICGA ved haleveneinjektion i AMD forskning ved hjælp af vores HTRA1 musemodel. Vores data tyder på, atIV-ICGA er overlegen i forhold til IP (eller subkutan (SC))-ICGA, der i øjeblikket anvendes inden for området 17,18 til karakterisering læsioner i årehinden. Erklæring om Animal Research Dyreforsøg blev udført i henhold til protokoller, der er godkendt af Institutional Animal Care og brug Udvalg (IACUC), og blev udført i overensstemmelse med ARVO erklæring til brug af dyr i Ophthalmic og Vision Research.

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			<td height="50" style="height:50px;width:216px;">Spectralis Multi-Modality Imaging System</td>
			<td style="width:152px;">Heidelberg Engineering, Germany</td>
			<td style="width:132px;">SPECTRALIS HRA+OCT</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Tropicamide ophthalmic solution (1%)</td>
			<td style="width:152px;">Bausch &#38; Lomb</td>
			<td style="width:132px;">NDC 24208-585-64</td>
			<td>for dilation of pupils</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">GenTeal Gel</td>
			<td style="width:152px;">Genteal</td>
			<td style="width:132px;">NDC 58768-791-15</td>
			<td>&#160;clear lubricant eye gel&#160;</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Ketamine</td>
			<td style="width:152px;">Vedco Inc</td>
			<td style="width:132px;">NDC 50989-996-06</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Xylazine</td>
			<td style="width:152px;">Lloyd Laboratories</td>
			<td style="width:132px;">NADA 139-236</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Acepromazine</td>
			<td style="width:152px;">Vedco Inc</td>
			<td style="width:132px;">NDC 50989-160-11</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">32-G Needle</td>
			<td>Steriject</td>
			<td style="width:132px;">PRE-32013</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">1-ml syringe</td>
			<td style="width:152px;">BD</td>
			<td style="width:132px;">309659</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Indocyanine Green</td>
			<td style="width:152px;">Pfaltz &#38; Bauer</td>
			<td style="width:132px;">I01250</td>
			<td></td>
		</tr>
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detecting abnormalities in choroidal vasculature in a mouse model of age-related macular degeneration by time-course indocyanine green angiography

Indocyaningrøn angiografi (eller ICGA) er en teknik udføres af øjenlæger at diagnosticere abnormiteter i choroidal og retinal vaskulatur af forskellige øjensygdomme såsom aldersrelateret makulær degeneration (AMD). ICGA er især nyttig til billede bageste koroidal vaskulatur i øjet på grund af sin evne til at trænge igennem det pigmenterede lag med sit infrarøde spektrum. ICGA tidsforløb kan opdeles i begyndelsen, midten og sene faser. De tre faser give værdifulde oplysninger om patologi øjenproblemer. Selvom tiden-retters ICGA ved intravenøs (IV) injektion er meget udbredt i klinikken for diagnosticering og behandling af choroide problemer, ICGA ved intraperitoneal injektion (IP) er almindeligt anvendt i dyreforsøg. Her har vi vist den teknik til at opnå høj opløsning ICGA tid-retters billederne i mus ved hale-vene indsprøjtning og konfokal scanning laser oftalmoskopi. Vi brugte denne teknik til billede choroidale lemelser i en musemodel af aldersrelateret makuladegeneration. Selv om det er meget lettere at introducere ICG til musen vasculature af IP, tyder vore data, at det er vanskeligt at opnå reproducerbare ICGA tidsforløb billeder ved IP-ICGA. I modsætning hertil ICGA via haleveneinjektion giver høj kvalitet ICGA tid-retters billeder sammenlignes med humane studier. Desuden viste vi, at ICGA udført på albino mus giver klarere billeder af choroidale fartøjer end der udføres på pigmenterede mus. Vi foreslår, at tidsforløbet IV-ICGA bør blive en almindelig praksis i AMD forskning baseret på dyremodeller.

Vi udførte ICGA tidsforløb i HTRA1 transgene mus og kontrol WT søskende, som begge er på CD1 baggrunden. Albino CD1 baggrund blev udvalgt til at lette indocyaningrønt angiografi (ICGA) imaging (se diskussionen). Nogle aneurism ligesom dilations begyndte at dukke op i den tidlige fase i HTRA1 mus (figur 2, en rød pil viser dilatation på spidsen af et skib og en rød cirkel angiver en klynge typen polypoidal læsion). Choroidale skibe er tydeligt i både WT og HTRA1 mus under denne tidlige fill-in ...

Watch this Scientific Journal Video about Detecting Abnormalities in Choroidal Vasculature in a Mouse Model of Age-related Macular Degeneration by Time-course Indocyanine Green Angiography at JoVE.com

Detecting Abnormalities in Choroidal Vasculature in a Mouse Model of Age-related Macular Degeneration by Time-course Indocyanine Green Angiography

Indocyaningrønt angiografi (eller ICGA) udført af haleveneinjektion leverer høj kvalitet ICGA tidsforløb billeder til at karakterisere abnormaliteter i mus årehinden.

Afsløring Abnormiteter i Choroidal karrene i en musemodel af Aldersrelateret makuladegeneration af Time-kursus indocyaningrønt angiografi

Indocyanine Green&#160;Angiography (or ICGA) is a technique performed by ophthalmologists to diagnose abnormalities of the choroidal and retinal ...

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Necrotizing enterocolitis (NEC) is the most severe gastrointestinal (GI) disease that often occurs in premature infants, especially very low birth weight ...

Robotic Left Hepatectomy using Indocyanine Green Fluorescence Imaging for an Intrahepatic Complex Biliary Cyst

Robotisk venstre hepatektomi ved hjælp af indocyanin grøn fluorescensbilleddannelse til en intrahepatisk kompleks galdecyste

Biliary cysts (BC) are rare congenital dilatations of intra- and extrahepatic parts of the biliary tract and bear a significant risk of carcinogenesis. ...

Fluorescent Laparoscopic Central Hepatectomy for Liver Cancer Using Indocyanine Green Negative Staining

Fluorescerende laparoskopisk central hepatektomi til leverkræft ved hjælp af indocyaningrøn negativ farvning

Laparoscopic hepatectomy is an important treatment method for liver cancer. In the past, the resection boundary was usually determined by intraoperative ...