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DOI :
10.3791/51087-v
•
9:37 min
August 15th, 2014
Chapters
0:05
Title
1:56
Preparations
3:42
Optimized Negative Staining
6:16
EM-Grid Analysis
7:18
Examples of Optimized Negative Staining (OpNS)
8:44
Conclusion
以上蛋白质的一半是小分子蛋白(分子量<200 kDa)的具有挑战,为电子显微镜成像和三维重建。优化的负染色是一个强大的和高通量的协议来获得高对比度和相对较高的分辨率(〜1毫微米)的小的非对称的蛋白质或复合物的不同生理条件下的图像。
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