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DOI :
10.3791/51088-v
•
12:25 min
January 7th, 2014
Chapters
0:05
Title
2:38
Single Cell GBM (Glioblastoma) Suspension for Neurosphere Culture
5:13
Morphological and Molecular Analysis of Neurospheres by Immunohistochemistry
8:35
Results: Representative Xenograft Tumor Formation from High Grade Glioma Cell Cultures
11:12
Conclusion
在无血清神经球介质中分离出的高档胶质瘤手术标本的传播协议,为具有癌症干细胞表型的细胞进行选择。对于不能作为神经圈生长的标本,建议采取替代方案。描述了一种石蜡嵌入技术,用于维持免疫细胞化学的3D神经球结构。
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