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DOI :
10.3791/51093-v
July 20th, 2014
Chapters
0:05
Title
1:31
Setup: The Day Before Tumor Harvest
2:21
Setup and Tumor Harvest
4:08
Tissue Dissociation and Trituration
7:59
Cell Plating
9:19
Results: Primary Vestibular Schwannoma Cultures
10:18
Conclusion
前庭神经鞘瘤(VSS)是雪旺细胞(SC)的原点非恶性肿瘤,与在NF2的肿瘤抑制基因的突变相关联。我们提出一个可重复的,高效的协议,用于原代人类VS细胞培养,允许分子和细胞实验操作和分析,概括人类疾病的异质性。
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