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DOI :
10.3791/51095-v
•
10:07 min
February 22nd, 2014
Chapters
0:05
Title
1:37
N. benthamiana and A. thaliana Protein Samples
3:24
Protein Immunoprecipitation
5:32
Protein Digestion
7:48
Results: Identification of Post-translational Modifications of Tagged Proteins
9:13
Conclusion
我们在这里描述的一个协议,用于植物蛋白质复合物的纯化和表征。我们证明了在一个复杂的免疫沉淀一个单一的蛋白质,这样我们就可以识别其翻译后修饰及其相互作用的合作伙伴。
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