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DOI :
10.3791/51095-v
•
10:07 min
February 22nd, 2014
Chapters
0:05
Title
1:37
N. benthamiana and A. thaliana Protein Samples
3:24
Protein Immunoprecipitation
5:32
Protein Digestion
7:48
Results: Identification of Post-translational Modifications of Tagged Proteins
9:13
Conclusion
ここでは植物性タンパク質複合体の精製および特性評価のためのプロトコルを記述します。私たちは、複雑な内部に単一のタンパク質を免疫沈降によって、私たちはその翻訳後修飾とその相互作用パートナーを識別できることを実証している。
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